FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2786688711> ?p ?o ?g. }

• W2786688711 abstract "TDP-43 is an RNA-binding protein active in splicing that concentrates into membraneless ribonucleoprotein granules and forms aggregates in amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Although best known for its predominantly disordered C-terminal domain which mediates ALS inclusions, TDP-43 has a globular N-terminal domain (NTD). Here, we show that TDP-43 NTD assembles into head-to-tail linear chains and that phosphomimetic substitution at S48 disrupts TDP-43 polymeric assembly, discourages liquid-liquid phase separation (LLPS) in vitro, fluidizes liquid-liquid phase separated nuclear TDP-43 reporter constructs in cells, and disrupts RNA splicing activity. Finally, we present the solution NMR structure of a head-to-tail NTD dimer comprised of two engineered variants that allow saturation of the native polymerization interface while disrupting higher-order polymerization. These data provide structural detail for the established mechanistic role of the well-folded TDP-43 NTD in splicing and link this function to LLPS. In addition, the fusion-tag solubilized, recombinant form of TDP-43 full-length protein developed here will enable future phase separation and in vitro biochemical assays on TDP-43 function and interactions that have been hampered in the past by TDP-43 aggregation." @default.
• W2786688711 created "2018-02-23" @default.
• W2786688711 creator A5001615371 @default.
• W2786688711 creator A5003043392 @default.
• W2786688711 creator A5009116370 @default.
• W2786688711 creator A5011026810 @default.
• W2786688711 creator A5021364495 @default.
• W2786688711 creator A5045689752 @default.
• W2786688711 creator A5045953252 @default.
• W2786688711 creator A5048172532 @default.
• W2786688711 creator A5048298487 @default.
• W2786688711 creator A5051136558 @default.
• W2786688711 creator A5055546235 @default.
• W2786688711 creator A5058009413 @default.
• W2786688711 creator A5074229775 @default.
• W2786688711 creator A5077689800 @default.
• W2786688711 creator A5085540282 @default.
• W2786688711 date "2018-02-09" @default.
• W2786688711 modified "2023-10-16" @default.
• W2786688711 title "A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing" @default.
• W2786688711 cites W1516409471 @default.
• W2786688711 cites W1756228642 @default.
• W2786688711 cites W1854281301 @default.
• W2786688711 cites W1938908576 @default.
• W2786688711 cites W1952608303 @default.
• W2786688711 cites W1961668975 @default.
• W2786688711 cites W1967443709 @default.
• W2786688711 cites W1967932847 @default.
• W2786688711 cites W1975317507 @default.
• W2786688711 cites W1979496072 @default.
• W2786688711 cites W1982021060 @default.
• W2786688711 cites W1983614185 @default.
• W2786688711 cites W1984145170 @default.
• W2786688711 cites W199186236 @default.
• W2786688711 cites W1995635963 @default.
• W2786688711 cites W1996174799 @default.
• W2786688711 cites W1997251321 @default.
• W2786688711 cites W1998591058 @default.
• W2786688711 cites W2003618511 @default.
• W2786688711 cites W2011387974 @default.
• W2786688711 cites W2012587917 @default.
• W2786688711 cites W2012840643 @default.
• W2786688711 cites W2020159880 @default.
• W2786688711 cites W2026226960 @default.
• W2786688711 cites W2026247887 @default.
• W2786688711 cites W2031964319 @default.
• W2786688711 cites W2035954330 @default.
• W2786688711 cites W2039385533 @default.
• W2786688711 cites W2041451000 @default.
• W2786688711 cites W2043274355 @default.
• W2786688711 cites W2047850449 @default.
• W2786688711 cites W2054352933 @default.
• W2786688711 cites W2057524757 @default.
• W2786688711 cites W2060211017 @default.
• W2786688711 cites W2061485287 @default.
• W2786688711 cites W2066952414 @default.
• W2786688711 cites W2081359386 @default.
• W2786688711 cites W2081420723 @default.
• W2786688711 cites W2081589139 @default.
• W2786688711 cites W2085978470 @default.
• W2786688711 cites W2088231318 @default.
• W2786688711 cites W2106390970 @default.
• W2786688711 cites W2110036405 @default.
• W2786688711 cites W2112418305 @default.
• W2786688711 cites W2115887554 @default.
• W2786688711 cites W2117428493 @default.
• W2786688711 cites W2119555549 @default.
• W2786688711 cites W2120156669 @default.
• W2786688711 cites W2124670830 @default.
• W2786688711 cites W2132166526 @default.
• W2786688711 cites W2136949432 @default.
• W2786688711 cites W2139066576 @default.
• W2786688711 cites W2150860983 @default.
• W2786688711 cites W2154923417 @default.
• W2786688711 cites W2157658519 @default.
• W2786688711 cites W2160311330 @default.
• W2786688711 cites W2164725970 @default.
• W2786688711 cites W2165962849 @default.
• W2786688711 cites W2166305185 @default.
• W2786688711 cites W2168386087 @default.
• W2786688711 cites W2168592676 @default.
• W2786688711 cites W2168639701 @default.
• W2786688711 cites W2169821755 @default.
• W2786688711 cites W2170671445 @default.
• W2786688711 cites W2237498135 @default.
• W2786688711 cites W2255386862 @default.
• W2786688711 cites W2283476610 @default.
• W2786688711 cites W2333401974 @default.
• W2786688711 cites W2345531090 @default.
• W2786688711 cites W2395887422 @default.
• W2786688711 cites W2400981255 @default.
• W2786688711 cites W2402791934 @default.
• W2786688711 cites W2481938983 @default.
• W2786688711 cites W2510545519 @default.
• W2786688711 cites W2587224453 @default.
• W2786688711 cites W2594130445 @default.
• W2786688711 cites W2610566121 @default.
• W2786688711 cites W2617973969 @default.
• W2786688711 cites W2649090908 @default.
• W2786688711 cites W2734358017 @default.

• next