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• W2787000623 endingPage "e1007223" @default.
• W2787000623 startingPage "e1007223" @default.
• W2787000623 abstract "Histone H3K4 methylation is a feature of meiotic recombination hotspots shared by many organisms including plants and mammals. Meiotic recombination is initiated by programmed double-strand break (DSB) formation that in budding yeast takes place in gene promoters and is promoted by histone H3K4 di/trimethylation. This histone modification is recognized by Spp1, a PHD finger containing protein that belongs to the conserved histone H3K4 methyltransferase Set1 complex. During meiosis, Spp1 binds H3K4me3 and interacts with a DSB protein, Mer2, to promote DSB formation close to gene promoters. How Set1 complex- and Mer2- related functions of Spp1 are connected is not clear. Here, combining genome-wide localization analyses, biochemical approaches and the use of separation of function mutants, we show that Spp1 is present within two distinct complexes in meiotic cells, the Set1 and the Mer2 complexes. Disrupting the Spp1-Set1 interaction mildly decreases H3K4me3 levels and does not affect meiotic recombination initiation. Conversely, the Spp1-Mer2 interaction is required for normal meiotic recombination initiation, but dispensable for Set1 complex-mediated histone H3K4 methylation. Finally, we provide evidence that Spp1 preserves normal H3K4me3 levels independently of the Set1 complex. We propose a model where Spp1 works in three ways to promote recombination initiation: first by depositing histone H3K4 methylation (Set1 complex), next by reading and protecting histone H3K4 methylation, and finally by making the link with the chromosome axis (Mer2-Spp1 complex). This work deciphers the precise roles of Spp1 in meiotic recombination and opens perspectives to study its functions in other organisms where H3K4me3 is also present at recombination hotspots." @default.
• W2787000623 created "2018-02-23" @default.
• W2787000623 creator A5005646934 @default.
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• W2787000623 date "2018-02-14" @default.
• W2787000623 modified "2023-10-17" @default.
• W2787000623 title "The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes" @default.
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• W2787000623 doi "https://doi.org/10.1371/journal.pgen.1007223" @default.
• W2787000623 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5828529" @default.
• W2787000623 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29444071" @default.
• W2787000623 hasPublicationYear "2018" @default.
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