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- W2787020869 abstract "本研究采用超高效液相色谱-三重四级杆串联质谱技术，建立在肿瘤细胞中测定单硫键连接的多柔比星前药（DOX-S-DOX）浓度的方法学，并应用于人乳腺癌细胞（MCF-7）的体外药代动力学研究。细胞样品前处理采用乙腈沉淀蛋白的方法。以柔红霉素为内标，采用反相C18色谱柱（Agilent Eclipse plus C18 RRHD 1.8 μm，2.1 mm×50 mm），流动相为甲醇-0.1%甲酸水溶液，以0.4 mL·min-1的流速进行梯度洗脱，每个样品的分析时间为6.0 min，样品经电喷雾离子源正离子化后，在多反应监测模式下测定DOX-S-DOX（m/z 1251.3→459.4）的浓度。线性范围为20.0~400 ng·mL-1（r2 ≥ 0.99）。高、中、低三个浓度质控样本的准确度（RE%）在-2.04%~2.62%之间，批内、批间精密度（RSD%）均在3.77%~8.35%以内。基质效应在104.8%~113.9%之间，提取回收率在97.67%~104.2%之间。该分析方法较为简便，其线性范围和灵敏度能够满足DOX-S-DOX（10 μg·mL-1）在MCF-7细胞中的药代动力学研究。" @default.
- W2787020869 created "2018-02-23" @default.
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- W2787020869 date "2017-10-19" @default.
- W2787020869 modified "2023-09-24" @default.
- W2787020869 title "UPLC-MS/MS法分析新型多柔比星前药在肿瘤细胞中的浓度" @default.
- W2787020869 doi "https://doi.org/10.16438/j.0513-4870.2017-1031" @default.
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