FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2787185087> ?p ?o ?g. }

• W2787185087 endingPage "350" @default.
• W2787185087 startingPage "340" @default.
• W2787185087 abstract "Abstract Coagulation factor XI is activated by thrombin or factor XIIa resulting in a conformational change that converts the catalytic domain into its active form and exposing exosites for factor IX on the apple domains. Although crystal structures of the zymogen factor XI and the catalytic domain of the protease are available, the structure of the apple domains and hence the interactions with the catalytic domain in factor XIa are unknown. We now used chemical footprinting to identify lysine residue containing regions that undergo a conformational change following activation of factor XI. To this end, we employed tandem mass tag in conjunction with mass spectrometry. Fifty-two unique peptides were identified, covering 37 of the 41 lysine residues present in factor XI. Two identified lysine residues that showed altered flexibility upon activation were mutated to study their contribution in factor XI stability or enzymatic activity. Lys357, part of the connecting loop between A4 and the catalytic domain, was more reactive in factor XIa but mutation of this lysine residue did not impact on factor XIa activity. Lys516 and its possible interactor Glu380 are located in the catalytic domain and are covered by the activation loop of factor XIa. Mutating Glu380 enhanced Arg369 cleavage and thrombin generation in plasma. In conclusion, we have identified novel regions that undergo a conformational change following activation. This information improves knowledge about factor XI and will contribute to development of novel inhibitors or activators for this coagulation protein." @default.
• W2787185087 created "2018-02-23" @default.
• W2787185087 creator A5018578667 @default.
• W2787185087 creator A5026484761 @default.
• W2787185087 creator A5033503892 @default.
• W2787185087 creator A5041501206 @default.
• W2787185087 creator A5042624694 @default.
• W2787185087 creator A5074112020 @default.
• W2787185087 date "2018-01-01" @default.
• W2787185087 modified "2023-09-27" @default.
• W2787185087 title "Chemical Footprinting Reveals Conformational Changes Following Activation of Factor XI" @default.
• W2787185087 cites W1493020617 @default.
• W2787185087 cites W1518067964 @default.
• W2787185087 cites W1524686766 @default.
• W2787185087 cites W1540279731 @default.
• W2787185087 cites W1894368067 @default.
• W2787185087 cites W1966037532 @default.
• W2787185087 cites W1971682587 @default.
• W2787185087 cites W1978820185 @default.
• W2787185087 cites W1980630446 @default.
• W2787185087 cites W1985407614 @default.
• W2787185087 cites W1987923388 @default.
• W2787185087 cites W1998761138 @default.
• W2787185087 cites W2000560444 @default.
• W2787185087 cites W2002431523 @default.
• W2787185087 cites W2011150204 @default.
• W2787185087 cites W2016202631 @default.
• W2787185087 cites W2018082148 @default.
• W2787185087 cites W2021548175 @default.
• W2787185087 cites W2021703235 @default.
• W2787185087 cites W2021716138 @default.
• W2787185087 cites W2031677121 @default.
• W2787185087 cites W2033379255 @default.
• W2787185087 cites W2039430475 @default.
• W2787185087 cites W2040257513 @default.
• W2787185087 cites W2042154654 @default.
• W2787185087 cites W2061296837 @default.
• W2787185087 cites W2063706685 @default.
• W2787185087 cites W2072757169 @default.
• W2787185087 cites W2073707438 @default.
• W2787185087 cites W2094058604 @default.
• W2787185087 cites W2099540110 @default.
• W2787185087 cites W2119768870 @default.
• W2787185087 cites W2143914653 @default.
• W2787185087 cites W2332512334 @default.
• W2787185087 cites W2398306859 @default.
• W2787185087 cites W295511661 @default.
• W2787185087 cites W2976903704 @default.
• W2787185087 doi "https://doi.org/10.1160/th17-09-0676" @default.
• W2787185087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29378358" @default.
• W2787185087 hasPublicationYear "2018" @default.
• W2787185087 type Work @default.
• W2787185087 sameAs 2787185087 @default.
• W2787185087 citedByCount "6" @default.
• W2787185087 countsByYear W27871850872019 @default.
• W2787185087 countsByYear W27871850872022 @default.
• W2787185087 countsByYear W27871850872023 @default.
• W2787185087 crossrefType "journal-article" @default.
• W2787185087 hasAuthorship W2787185087A5018578667 @default.
• W2787185087 hasAuthorship W2787185087A5026484761 @default.
• W2787185087 hasAuthorship W2787185087A5033503892 @default.
• W2787185087 hasAuthorship W2787185087A5041501206 @default.
• W2787185087 hasAuthorship W2787185087A5042624694 @default.
• W2787185087 hasAuthorship W2787185087A5074112020 @default.
• W2787185087 hasConcept C104317684 @default.
• W2787185087 hasConcept C112516734 @default.
• W2787185087 hasConcept C118552586 @default.
• W2787185087 hasConcept C151730666 @default.
• W2787185087 hasConcept C15744967 @default.
• W2787185087 hasConcept C175156509 @default.
• W2787185087 hasConcept C181199279 @default.
• W2787185087 hasConcept C185592680 @default.
• W2787185087 hasConcept C186738567 @default.
• W2787185087 hasConcept C203014093 @default.
• W2787185087 hasConcept C2776016237 @default.
• W2787185087 hasConcept C2777292125 @default.
• W2787185087 hasConcept C2777339483 @default.
• W2787185087 hasConcept C2777444498 @default.
• W2787185087 hasConcept C2778021871 @default.
• W2787185087 hasConcept C2778382381 @default.
• W2787185087 hasConcept C2778775115 @default.
• W2787185087 hasConcept C2779036427 @default.
• W2787185087 hasConcept C2781338088 @default.
• W2787185087 hasConcept C2911038020 @default.
• W2787185087 hasConcept C43369102 @default.
• W2787185087 hasConcept C47701112 @default.
• W2787185087 hasConcept C515207424 @default.
• W2787185087 hasConcept C55493867 @default.
• W2787185087 hasConcept C71240020 @default.
• W2787185087 hasConcept C86339819 @default.
• W2787185087 hasConcept C86803240 @default.
• W2787185087 hasConcept C89560881 @default.
• W2787185087 hasConceptScore W2787185087C104317684 @default.
• W2787185087 hasConceptScore W2787185087C112516734 @default.
• W2787185087 hasConceptScore W2787185087C118552586 @default.
• W2787185087 hasConceptScore W2787185087C151730666 @default.
• W2787185087 hasConceptScore W2787185087C15744967 @default.
• W2787185087 hasConceptScore W2787185087C175156509 @default.

• next