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- W278749278 abstract "In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.Then the DNA fragment of bovine antibacterial peptide was amplified by RT-PCR and inserted into pET30a vector to construct pET30a-BNBD5.The fusion protein was expressed from pET30a-BNBD5 induced with IPTG and analyzed by Western-blot.The amplified DNA fragment 138 bp in size was identical to the BNBD5 sequence registered in GenBank.The cDNA fragment encoded 45 amino acid with 6 invariant cysteine residues.The expressed fusion protein was 10 ku in molecular mass,as was expected.The results confirmed that the BNBD5 protein was expressed successfully in Escherichia coli." @default.
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- W278749278 date "2009-01-01" @default.
- W278749278 modified "2023-09-28" @default.
- W278749278 title "Cloning and prokaryotic expression of endometrial antibacterial peptide BNBD5 gene of dairy cow." @default.
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