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- W2787612069 abstract "Porphyromonas gingivalis an anaerobic Gram- negative bacilli, is the major causative agent in the initiation and progression of severe forms of pe- riodontal disease. This organism is armed with a number of putative virulence factors, like the ability to adhere to periodontal tissues and other oral bacteria and Lipopolysaccharide (LPS) that induces the host immune response and multiple proteases. These molecular bases of virulence and their relationship to genetic diversity have not been sufficiently understood yet. The aim of the present study was to genotype isolates of P. gingivalis selected from patients with chronic and aggressive periodontitis, using a PCR- based methodology for the insertion sequence (IS1126). Subgingival plaque samples were collected from four affected sites (the site of greatest depth of periodontal pocket per quadrant). The samples were obtained by inserting sterile paper points and were pooled in cold RTF until processed in the laboratory. Black-pigmented bacteria for subsequent molecular, phenotypic and genotyping identification were obtained by cultivation of the confirmed isolates by PCR as P.gingivalis. A total of 35 isolates were subjected to genotyping from 6 patients, and 7 different genotypes were identified. Four patients had a single genotype in their isolates, whereas 2 showed two distinct genetic profiles. In conclusion, the isolates of P. gingivalis studied showed genetic variability within the IS1126 sequence and perhaps because of the number of samples analyzed a bacterial gene profile characteristic of chronic or aggressive periodontitis was not identified. Rev. Clin. Periodoncia Implantol. Rehabil. Oral Vol. 2(2); 54-58, 2009." @default.
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- W2787612069 date "2009-01-01" @default.
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- W2787612069 title "Genotipificación de Porphyromonas gingivalis en Pacientes con Periodontitis Genotyping of Porphyromonas gingivalis in Patients with Periodontitis" @default.
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