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- W2787715566 abstract "Traditional bioproduct engineering focuses on pathway optimization, yet is often complicated by product inhibition, downstream consumption, and the toxicity of certain products. Here, we present the co-compartmentation of biosynthesis and storage via a synthetic droplet as an effective new strategy to improve the bioproduct yield, with squalene as a model compound. A hydrophobic protein was designed and introduced into the tobacco chloroplast to generate a synthetic droplet for terpene storage. Simultaneously, squalene biosynthesis enzymes were introduced to chloroplasts together with the droplet-forming protein to co-compartmentalize the biosynthesis and storage of squalene. The strategy has enabled a record yield of squalene at 2.6 mg/g fresh weight without compromising plant growth. Confocal fluorescent microscopy imaging, stimulated Raman scattering microscopy, and droplet composition analysis confirmed the formation of synthetic storage droplet in chloroplast. The co-compartmentation of synthetic storage droplet with a targeted metabolic pathway engineering represents a new strategy for enhancing bioproduct yield." @default.
- W2787715566 created "2018-02-23" @default.
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- W2787715566 date "2018-02-13" @default.
- W2787715566 modified "2023-10-05" @default.
- W2787715566 title "Co-Compartmentation of Terpene Biosynthesis and Storage <i>via</i> Synthetic Droplet" @default.
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- W2787715566 doi "https://doi.org/10.1021/acssynbio.7b00368" @default.
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