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- W2787801715 abstract "Many chemotaxis assays allow for the assessment of bacterial chemotaxis by determining the number of cells migrating toward a chemoattractant or away from a chemorepellent. Some of these assays use a capillary filled with a chemoeffector/agarose mixture to allow cells to accumulate at the mouth of the capillary. Subsequently, assumptions about the relative strengths of chemotaxis strength are based on visual comparisons. Here, we describe a modification of this assay that uses a hydrogel matrix to enable quantitative time-course measurements by analyzing image pixel intensities. This approach allows a high-throughput method when coupled with the aid of a motorized microscope stage." @default.
- W2787801715 created "2018-02-23" @default.
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- W2787801715 date "2018-01-01" @default.
- W2787801715 modified "2023-10-16" @default.
- W2787801715 title "Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries" @default.
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- W2787801715 doi "https://doi.org/10.1007/978-1-4939-7577-8_4" @default.
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