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• W2788173399 abstract "Here, we demonstrate that lens-free video microscopy enables us to simultaneously capture the kinetics of thousands of cells directly inside the incubator and that it is possible to monitor and quantify single cells along several cell cycles. We describe the full protocol used to monitor and quantify a HeLa cell culture for 2.7 days. First, cell culture acquisition is performed with a lens-free video microscope, and then the data is analyzed following a four-step process: multi-wavelength holographic reconstruction, cell-tracking, cell segmentation and cell division detection algorithms. As a result, we show that it is possible to gather a dataset featuring more than 10,000 cell cycle tracks and more than 2 x 106 cell morphological measurements." @default.
• W2788173399 created "2018-03-06" @default.
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• W2788173399 date "2018-02-23" @default.
• W2788173399 modified "2023-09-25" @default.
• W2788173399 title "Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture" @default.
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• W2788173399 doi "https://doi.org/10.3791/56580" @default.
• W2788173399 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5931341" @default.
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