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• W2788174495 abstract "Single-molecule real-time (SMRT) sequencing developed by PacBio, also called third-generation sequencing (TGS), offers longer reads than the second-generation sequencing (SGS). Given its ability to obtain full-length transcripts without assembly, isoform sequencing (Iso-Seq) of transcriptomes by PacBio is advantageous for genome annotation, identification of novel genes and isoforms, as well as the discovery of long non-coding RNA (lncRNA). In addition, Iso-Seq gives access to the direct detection of alternative splicing, alternative polyadenylation (APA), gene fusion, and DNA modifications. Such applications of Iso-Seq facilitate the understanding of gene structure, post-transcriptional regulatory networks, and subsequently proteomic diversity. In this review, we summarize its applications in plant transcriptome study, specifically pointing out challenges associated with each step in the experimental design and highlight the development of bioinformatic pipelines. We aim to provide the community with an integrative overview and a comprehensive guidance to Iso-Seq, and thus to promote its applications in plant research." @default.
• W2788174495 created "2018-03-06" @default.
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• W2788174495 date "2018-01-18" @default.
• W2788174495 modified "2023-09-28" @default.
• W2788174495 title "Isoform Sequencing and State-of-Art Applications for Unravelling Complexity of Plant Transcriptomes" @default.
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• W2788174495 doi "https://doi.org/10.3390/genes9010043" @default.
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• W2788174495 hasPublicationYear "2018" @default.
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