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- W2788280797 abstract "为了解 Sox 14基因在拟穴青蟹胚胎发育和性腺发育过程中可能起到的调控作用，实验从青蟹性腺转录组数据库中得到全长为2558 bp的 Sp-Sox 14 cDNA序列，该序列编码一个包含427个氨基酸的蛋白，包含一个HMG-box。进化树分析表明， Sp -Sox14与其他节肢动物的Sox14亲缘关系较近。实时定量PCR结果显示， Sp-Sox 14在成蟹雌雄各组织中均有不同程度的表达，其中在卵巢和雄蟹心脏中的表达量最高。在胚胎发育过程中， Sp-Sox 14在复眼色素形成期的表达量显著高于其他时期。在性腺不同发育阶段， Sp-Sox 14在卵黄发生前期(O2)的表达量显著高于卵巢其他发育阶段；而在精巢发育过程中，其表达量在成熟精子期(T3)高于精母细胞期(T1)和精子细胞期(T2)。推测其参与卵巢的前期发育以及精子成熟等过程。整胚原位杂交结果显示， Sp-Sox 14在青蟹胚胎复眼色素形成期阳性信号定位于头部以及鄂足附近，在近孵化期定位于复眼附近，幼体孵出期则在头部仍有少量信号，暗示其与青蟹神经器官的形成以及体节附肢的发生有关。" @default.
- W2788280797 created "2018-03-06" @default.
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- W2788280797 date "2018-02-25" @default.
- W2788280797 modified "2023-09-23" @default.
- W2788280797 title "拟穴青蟹 Sp-Sox 14基因在胚胎发育和性腺发育过程中的表达" @default.
- W2788280797 doi "https://doi.org/10.11964/jfc.20170210712" @default.
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