FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2788606595> ?p ?o ?g. }

• W2788606595 endingPage "e0191489" @default.
• W2788606595 startingPage "e0191489" @default.
• W2788606595 abstract "Stem cells derived from placental tissues are an attractive source of cells for regenerative medicine. Amniotic epithelial cells isolated from human amnion (hAECs) have desirable and competitive characteristics that make them stand out between other stem cells. They have the ability to differentiate toward all three germ layers, they are not tumorigenic and they have immunosuppressive properties. Although liver transplantation is the best way to treat acute and chronic hepatic failure patients, there are several obstacles. Recently, stem cells have been spotlighted as alternative source of hepatocytes because of their potential for hepatogenic differentiation. In this work, we aimed to study the proliferation and survival of the hAECs during their hepatic differentiation. We have also analyzed the changes in pluripotency and hepatic markers. We differentiated amniotic cells applying a specific hepatic differentiation (HD) protocol. We determined by qRT-PCR that hAECs express significant levels of SOX-2, OCT-4 and NANOG during at least 15 days in culture and these pluripotent markers diminish during HD. SSEA-4 expression was reduced during HD, measured by immunofluorescence. Morphological characteristics became more similar to hepatic ones in differentiated cells and representative hepatic markers significantly augmented their expression, measured by qRT-PCR and Western blot. Cells achieved a differentiation efficiency of 75%. We observed that HD induced proliferation and promoted survival of hAECs, during 30 days in culture, evaluated by 3H-thymidine incorporation and MTT assay. HD also promoted changes in hAECs cell cycle. Cyclin D1 expression increased, while p21 and p53 levels were reduced. Immunofluorescence analysis showed that Ki-67 expression was upregulated during HD. Finally, ERK 1/2 phosphorylation, which is intimately linked to proliferation and cell survival, augmented during all HD process and the inhibition of this signaling pathway affected not only proliferation but also differentiation. Our results suggest that HD promotes proliferation and survival of hAECs, providing important evidence about the mechanisms governing their hepatic differentiation. We bring new knowledge concerning some of the optimal transplantation conditions for these hepatic like cells." @default.
• W2788606595 created "2018-03-06" @default.
• W2788606595 creator A5039909627 @default.
• W2788606595 creator A5042580806 @default.
• W2788606595 creator A5042939106 @default.
• W2788606595 creator A5051790848 @default.
• W2788606595 creator A5053049274 @default.
• W2788606595 creator A5053516175 @default.
• W2788606595 creator A5054194470 @default.
• W2788606595 creator A5065411322 @default.
• W2788606595 creator A5090038775 @default.
• W2788606595 date "2018-01-18" @default.
• W2788606595 modified "2023-10-17" @default.
• W2788606595 title "Proliferation and survival of human amniotic epithelial cells during their hepatic differentiation" @default.
• W2788606595 cites W1165742044 @default.
• W2788606595 cites W125913459 @default.
• W2788606595 cites W1488721352 @default.
• W2788606595 cites W1492509640 @default.
• W2788606595 cites W1544512904 @default.
• W2788606595 cites W1580549116 @default.
• W2788606595 cites W1581886960 @default.
• W2788606595 cites W1664417644 @default.
• W2788606595 cites W1849819493 @default.
• W2788606595 cites W1958123959 @default.
• W2788606595 cites W1966758968 @default.
• W2788606595 cites W196685199 @default.
• W2788606595 cites W1967000850 @default.
• W2788606595 cites W1976802994 @default.
• W2788606595 cites W1980216307 @default.
• W2788606595 cites W1983739891 @default.
• W2788606595 cites W1985139403 @default.
• W2788606595 cites W1986875558 @default.
• W2788606595 cites W1988115748 @default.
• W2788606595 cites W1991176399 @default.
• W2788606595 cites W1993863744 @default.
• W2788606595 cites W1994814948 @default.
• W2788606595 cites W2001344472 @default.
• W2788606595 cites W2004247606 @default.
• W2788606595 cites W2006020471 @default.
• W2788606595 cites W2008039855 @default.
• W2788606595 cites W2009243547 @default.
• W2788606595 cites W2010233175 @default.
• W2788606595 cites W2010608530 @default.
• W2788606595 cites W2011032636 @default.
• W2788606595 cites W2013153771 @default.
• W2788606595 cites W2015050458 @default.
• W2788606595 cites W2015985384 @default.
• W2788606595 cites W2016165075 @default.
• W2788606595 cites W2016492849 @default.
• W2788606595 cites W2018643417 @default.
• W2788606595 cites W2018896819 @default.
• W2788606595 cites W2024431976 @default.
• W2788606595 cites W2026684783 @default.
• W2788606595 cites W2027540026 @default.
• W2788606595 cites W2031789381 @default.
• W2788606595 cites W2033323307 @default.
• W2788606595 cites W2036159078 @default.
• W2788606595 cites W2038113465 @default.
• W2788606595 cites W2039488381 @default.
• W2788606595 cites W2039502780 @default.
• W2788606595 cites W2041795983 @default.
• W2788606595 cites W2053653008 @default.
• W2788606595 cites W2056158473 @default.
• W2788606595 cites W2057382495 @default.
• W2788606595 cites W2062101834 @default.
• W2788606595 cites W2062985668 @default.
• W2788606595 cites W2066468135 @default.
• W2788606595 cites W2067587938 @default.
• W2788606595 cites W2070991874 @default.
• W2788606595 cites W2071196172 @default.
• W2788606595 cites W2071753279 @default.
• W2788606595 cites W2072001178 @default.
• W2788606595 cites W2072239523 @default.
• W2788606595 cites W2075641639 @default.
• W2788606595 cites W2077264573 @default.
• W2788606595 cites W2077547257 @default.
• W2788606595 cites W2078240388 @default.
• W2788606595 cites W2078918257 @default.
• W2788606595 cites W2081736574 @default.
• W2788606595 cites W2082624253 @default.
• W2788606595 cites W2085292554 @default.
• W2788606595 cites W2085491292 @default.
• W2788606595 cites W2086096515 @default.
• W2788606595 cites W2086943348 @default.
• W2788606595 cites W2088088839 @default.
• W2788606595 cites W2089997622 @default.
• W2788606595 cites W2094557730 @default.
• W2788606595 cites W2097386209 @default.
• W2788606595 cites W2102284755 @default.
• W2788606595 cites W2102679822 @default.
• W2788606595 cites W2106406104 @default.
• W2788606595 cites W2107277218 @default.
• W2788606595 cites W2121197326 @default.
• W2788606595 cites W2121676829 @default.
• W2788606595 cites W2125395000 @default.
• W2788606595 cites W2126486896 @default.
• W2788606595 cites W2126605105 @default.
• W2788606595 cites W2128508184 @default.

• next