FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2788645059> ?p ?o ?g. }

• W2788645059 abstract "An increasing number of cyclic dipeptides (CDPs) have been shown to exhibit important biological activity including antifungal, antibacterial, anticonvulsant and immunomodulatory activity. Furthermore, some CDP derivatives have been shown to exhibit antitumour activity in vitro and in vivo. Several proline-based CDPs that exhibit biological activity have been detected in various processed foods and beverages. In the present study, the potential of seven proline-based CDPs to inhibit cancer cell growth was investigated in HT-29 (colon), HeLa (cervical), MCF-7 (breast) and WHCO3 (oesophageal) cancer cell lines. The CDPs used in this study were cyclo(Phe-Pro), cyclo(Tyr-Pro), cyclo(Gly-Pro), cyclo(Pro- Pro), cyclo(His-Pro), cyclo(Leu-Pro) and cyclo(Thr-Pro). The sulforhodamine B (SRB) cell growth assay was used in an initial screening phase to investigate the effects of the CDPs in HT-29, HeLa and MCF-7 cells. After exposing the cells to 10mM of the respective CDPs for 48 hours, the SRB assay results showed that only cyclo(Phe-Pro) exhibited more than 50% growth inhibition (p<0.01) in the three cell lines. The other CDPs showed comparatively marginal growth-inhibitory effects, except for cyclo(Tyr-Pro), which exhibited a pronounced effect in MCF-7 cells compared to HT-29 and HeLa cells. The MTT assay was used to confirm the SRB assay results for cyclo(Phe-Pro) and cyclo(Tyr-Pro), extending the investigation to the use of the fourth cell line WHCO3 and using a longer exposure time of 72 hours. The MTT assay demonstrated a dosedependent (0.008-10 mM) growth inhibition by cyclo(Phe-Pro) with an IC50 value of 4.04 ± 1.15 mM for HT-29 cells. Cyclo(Phe-Pro) was subsequently used to investigate whether the growth-inhibitory effects of this CDP were related to the induction of apoptosis in HT-29 cells. Hoechst 33342 staining showed that 5mM cyclo(Phe-Pro) induced characteristic chromatin condensation and nuclear fragmentation in 18.3 ± 2.8% (p<0.01) of HT-29 cells after 72 hours. Furthermore, annexin V binding revealed that HT-29 cells treated with 5 mM cyclo(Phe-Pro) displayed phosphatidylserine externalization after 48 hours. In addition, it was shown that 10 mM cyclo(Phe-Pro) induced poly(ADP-ribose)polymerase PARP cleavage, one of the hallmark events of apoptosis. The use of the broad-range caspase inhibitor Z-VAD-FMK, showed that this PARP cleavage was caspase-dependent, which in turn was confirmed by demonstrating an increase in caspase-3 activity (p<0.01) in cyclo(Phe- Pro)-treated HT-29 cells. In conclusion, these findings demonstrate that cyclo(Phe-Pro) inhibited the growth of HT- 29, MCF-7, HeLa and WHCO3 cells, and induced apoptosis in HT-29 colon cancer cells, suggesting the potential antitumour activity of cyclo(Phe-Pro)-related CDPs." @default.
• W2788645059 created "2018-03-06" @default.
• W2788645059 creator A5007288168 @default.
• W2788645059 date "2004-01-01" @default.
• W2788645059 modified "2023-09-27" @default.
• W2788645059 title "The effects of selected proline-based cyclic dipeptides on growth and induction of apoptosis in cancer cells" @default.
• W2788645059 cites W132061808 @default.
• W2788645059 cites W142053782 @default.
• W2788645059 cites W1483201954 @default.
• W2788645059 cites W1496932838 @default.
• W2788645059 cites W1505801452 @default.
• W2788645059 cites W1528365264 @default.
• W2788645059 cites W1536544150 @default.
• W2788645059 cites W1579963067 @default.
• W2788645059 cites W1586081494 @default.
• W2788645059 cites W1589415602 @default.
• W2788645059 cites W1594804854 @default.
• W2788645059 cites W1599775292 @default.
• W2788645059 cites W1745634660 @default.
• W2788645059 cites W1832181040 @default.
• W2788645059 cites W1847565384 @default.
• W2788645059 cites W1931140155 @default.
• W2788645059 cites W1940692702 @default.
• W2788645059 cites W1942214720 @default.
• W2788645059 cites W1943174022 @default.
• W2788645059 cites W1963546793 @default.
• W2788645059 cites W1965023247 @default.
• W2788645059 cites W1976570855 @default.
• W2788645059 cites W1977600211 @default.
• W2788645059 cites W1978483631 @default.
• W2788645059 cites W1985148152 @default.
• W2788645059 cites W1988402636 @default.
• W2788645059 cites W1993224545 @default.
• W2788645059 cites W1993570389 @default.
• W2788645059 cites W1997005484 @default.
• W2788645059 cites W1997533132 @default.
• W2788645059 cites W1998332130 @default.
• W2788645059 cites W1999395478 @default.
• W2788645059 cites W2002584902 @default.
• W2788645059 cites W2003995825 @default.
• W2788645059 cites W2004487378 @default.
• W2788645059 cites W2005425364 @default.
• W2788645059 cites W2006238263 @default.
• W2788645059 cites W2008186920 @default.
• W2788645059 cites W2010298858 @default.
• W2788645059 cites W2013273130 @default.
• W2788645059 cites W2019327140 @default.
• W2788645059 cites W2028582699 @default.
• W2788645059 cites W2028893004 @default.
• W2788645059 cites W2033042511 @default.
• W2788645059 cites W2038753750 @default.
• W2788645059 cites W2039992040 @default.
• W2788645059 cites W2042850417 @default.
• W2788645059 cites W2043556557 @default.
• W2788645059 cites W2044324079 @default.
• W2788645059 cites W2045309540 @default.
• W2788645059 cites W2045992813 @default.
• W2788645059 cites W2049351910 @default.
• W2788645059 cites W2052853635 @default.
• W2788645059 cites W2054335548 @default.
• W2788645059 cites W2056212849 @default.
• W2788645059 cites W2057320402 @default.
• W2788645059 cites W2060048383 @default.
• W2788645059 cites W2061759533 @default.
• W2788645059 cites W2066861312 @default.
• W2788645059 cites W2069731715 @default.
• W2788645059 cites W2072894284 @default.
• W2788645059 cites W2075680556 @default.
• W2788645059 cites W207578865 @default.
• W2788645059 cites W2079051331 @default.
• W2788645059 cites W2081211388 @default.
• W2788645059 cites W2082259697 @default.
• W2788645059 cites W2083980483 @default.
• W2788645059 cites W2085418183 @default.
• W2788645059 cites W2086470568 @default.
• W2788645059 cites W2087092800 @default.
• W2788645059 cites W2090997246 @default.
• W2788645059 cites W2092818633 @default.
• W2788645059 cites W2094220461 @default.
• W2788645059 cites W2097202616 @default.
• W2788645059 cites W2101647884 @default.
• W2788645059 cites W2101698988 @default.
• W2788645059 cites W2107622792 @default.
• W2788645059 cites W2109689528 @default.
• W2788645059 cites W2119728044 @default.
• W2788645059 cites W2120064876 @default.
• W2788645059 cites W2122792830 @default.
• W2788645059 cites W2132818730 @default.
• W2788645059 cites W2139397808 @default.
• W2788645059 cites W2140927361 @default.
• W2788645059 cites W2143080883 @default.
• W2788645059 cites W2143484912 @default.
• W2788645059 cites W2144170214 @default.
• W2788645059 cites W2155208683 @default.
• W2788645059 cites W2206402902 @default.
• W2788645059 cites W2324554238 @default.
• W2788645059 cites W2398002362 @default.
• W2788645059 cites W2519542108 @default.
• W2788645059 cites W3198140284 @default.
• W2788645059 cites W611537134 @default.

• next