Matches in SemOpenAlex for { <https://semopenalex.org/work/W2788714489> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2788714489 abstract "Epigenetics regulate the gene expression while imposing no change in the underlying gene sequence constitution. The abnormal regulation of epigenetics is associated with the development and progression of cancer. Among the epigenetic regulators, UHRF1 (ubiquitin-like with PHD and ring finger domain containing protein 1) has attracted considerable attentions in cancer research in past years due to its universally increased expression in a wide range of different cancer cells, and its ability to facilitate the crosstalk between DNA methylation and histone modification, thereby driving the occurrence of cancer and ensuring the inheritance of accurate epigenomic information to descendent cells. Depletion of UHRF1 proteins in nuclear significantly blocked DNA replication in Xenopus egg extracts. However, the mechanism underlying the role of UHRF1 in DNA replication remains unclear. Considering the effects of UHRF1 depletion on DNA replication is independent of cell cycle checkpoints and transcription events in Xenopus egg extracts, it is important to extend the work into a higher eukaryote system. Therefore, we planned to generate UHRF1 knockout cell line in the most effective gene targeting system of DT40 cells to observe the effect of UHRF1 deletion on DNA replication and the cellular response to DNA damage. CRISPR/Cas9 technology and traditional gene targeting were applied to generate conditional UHRF1 knockout cell lines. It was found that knocking out the expression of both alleles of UHRF1 was lethal to cell viability and that there was a threshold of UHRF1 overexpression that can be tolerated by DT40 cells. Additionally, the ability of DT40 cells to tolerate DNA damage was positively related to the expression levels of UHRF1 proteins." @default.
- W2788714489 created "2018-03-06" @default.
- W2788714489 creator A5077784151 @default.
- W2788714489 date "2017-01-01" @default.
- W2788714489 modified "2023-09-23" @default.
- W2788714489 title "Disruption of UHRF1 expression in chicken DT40 cells" @default.
- W2788714489 hasPublicationYear "2017" @default.
- W2788714489 type Work @default.
- W2788714489 sameAs 2788714489 @default.
- W2788714489 citedByCount "0" @default.
- W2788714489 crossrefType "dissertation" @default.
- W2788714489 hasAuthorship W2788714489A5077784151 @default.
- W2788714489 hasConcept C104317684 @default.
- W2788714489 hasConcept C121912465 @default.
- W2788714489 hasConcept C143425029 @default.
- W2788714489 hasConcept C150194340 @default.
- W2788714489 hasConcept C153911025 @default.
- W2788714489 hasConcept C175193931 @default.
- W2788714489 hasConcept C190727270 @default.
- W2788714489 hasConcept C41091548 @default.
- W2788714489 hasConcept C54355233 @default.
- W2788714489 hasConcept C552990157 @default.
- W2788714489 hasConcept C73573662 @default.
- W2788714489 hasConcept C86803240 @default.
- W2788714489 hasConcept C95444343 @default.
- W2788714489 hasConcept C98086919 @default.
- W2788714489 hasConceptScore W2788714489C104317684 @default.
- W2788714489 hasConceptScore W2788714489C121912465 @default.
- W2788714489 hasConceptScore W2788714489C143425029 @default.
- W2788714489 hasConceptScore W2788714489C150194340 @default.
- W2788714489 hasConceptScore W2788714489C153911025 @default.
- W2788714489 hasConceptScore W2788714489C175193931 @default.
- W2788714489 hasConceptScore W2788714489C190727270 @default.
- W2788714489 hasConceptScore W2788714489C41091548 @default.
- W2788714489 hasConceptScore W2788714489C54355233 @default.
- W2788714489 hasConceptScore W2788714489C552990157 @default.
- W2788714489 hasConceptScore W2788714489C73573662 @default.
- W2788714489 hasConceptScore W2788714489C86803240 @default.
- W2788714489 hasConceptScore W2788714489C95444343 @default.
- W2788714489 hasConceptScore W2788714489C98086919 @default.
- W2788714489 hasLocation W27887144891 @default.
- W2788714489 hasOpenAccess W2788714489 @default.
- W2788714489 hasPrimaryLocation W27887144891 @default.
- W2788714489 hasRelatedWork W2022387696 @default.
- W2788714489 hasRelatedWork W2026550542 @default.
- W2788714489 hasRelatedWork W2044984526 @default.
- W2788714489 hasRelatedWork W2165877375 @default.
- W2788714489 hasRelatedWork W2253339144 @default.
- W2788714489 hasRelatedWork W2552964531 @default.
- W2788714489 hasRelatedWork W2572823110 @default.
- W2788714489 hasRelatedWork W2605892372 @default.
- W2788714489 hasRelatedWork W2734775561 @default.
- W2788714489 hasRelatedWork W2794608908 @default.
- W2788714489 hasRelatedWork W2803184601 @default.
- W2788714489 hasRelatedWork W2903366507 @default.
- W2788714489 hasRelatedWork W2908633943 @default.
- W2788714489 hasRelatedWork W2978223944 @default.
- W2788714489 hasRelatedWork W3022591994 @default.
- W2788714489 hasRelatedWork W3082645374 @default.
- W2788714489 hasRelatedWork W3125172187 @default.
- W2788714489 hasRelatedWork W3146854709 @default.
- W2788714489 hasRelatedWork W3192215732 @default.
- W2788714489 hasRelatedWork W613046234 @default.
- W2788714489 isParatext "false" @default.
- W2788714489 isRetracted "false" @default.
- W2788714489 magId "2788714489" @default.
- W2788714489 workType "dissertation" @default.