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- W2788954906 abstract "Researchers are always searching for novel biologically active molecules including peptides. With the improvement of equipment for electrospray mass spectrometry, it is now possible to identify hundreds of novel peptides in a single run. However, after identifying the peptide sequences it is expensive to synthesize all the peptides to perform biological activity assays. Here, we describe a substrate capture assay that uses inactive oligopeptidases to identify putative biologically active peptides in complexes peptide mixtures. This methodology can use any crude extracts of biological tissues or cells, with the advantage to introduce a filter (i.e., binding to an inactive oligopeptidase) as a prior step in screening to bioactive peptides." @default.
- W2788954906 created "2018-03-06" @default.
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- W2788954906 date "2018-01-01" @default.
- W2788954906 modified "2023-10-18" @default.
- W2788954906 title "Substrate Capture Assay Using Inactive Oligopeptidases to Identify Novel Peptides" @default.
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- W2788954906 doi "https://doi.org/10.1007/978-1-4939-7537-2_6" @default.
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- W2788954906 hasPublicationYear "2018" @default.
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