Matches in SemOpenAlex for { <https://semopenalex.org/work/W2788974715> ?p ?o ?g. }
- W2788974715 endingPage "263" @default.
- W2788974715 startingPage "256" @default.
- W2788974715 abstract "Abstract Actinidia arguta, with sweet flavour, high nutritional value and health promoting role, is a typical dioecious plant. But dioecy represents an important constraint in kiwifruit breeding programmes and also requires identification of male and female genotypes before planting an orchard. Therefore, understanding molecular basis of sex determination in A. arguta is important for molecular identification of seedling gender at early stage. PCR-based suppressive subtractive hybridization (SSH) was performed to screen genes related to sex determination. A total of 533 and 508 positive clones, from forward and reverse libraries respectively, were selected for sequencing. The clean ESTs were assembled into 309 (forwad) and 369 (reverse) unigenes, and 228 and 299 of them hit known homologous sequences of the NCBI nr database. Based on Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, four unigenes that might be involved in sex determination were selected for validation by qRT-PCR. The expression of PME was very strong in male flower at late development stage, with 72 times higher than that of female flower. Reversely, male flower exhibited extremely low expression level of ACO, MAN1 and MYC2 at late development stage as compared with female flower. The variable expression patterns of these genes between male and female flowers suggested their importance in controlling sex expression. These results advanced our understanding of the molecular mechanism of sex determination in A. arguta." @default.
- W2788974715 created "2018-03-06" @default.
- W2788974715 creator A5018863416 @default.
- W2788974715 creator A5025649021 @default.
- W2788974715 creator A5041416953 @default.
- W2788974715 creator A5047191501 @default.
- W2788974715 creator A5066886328 @default.
- W2788974715 creator A5069602593 @default.
- W2788974715 creator A5083275305 @default.
- W2788974715 date "2018-03-01" @default.
- W2788974715 modified "2023-09-22" @default.
- W2788974715 title "Identification and characterization of sex related genes in Actinidia arguta by suppression subtractive hybridization" @default.
- W2788974715 cites W1549163438 @default.
- W2788974715 cites W166139576 @default.
- W2788974715 cites W1910418210 @default.
- W2788974715 cites W1920163244 @default.
- W2788974715 cites W1950779100 @default.
- W2788974715 cites W1963655631 @default.
- W2788974715 cites W1979882976 @default.
- W2788974715 cites W1981133092 @default.
- W2788974715 cites W1986520383 @default.
- W2788974715 cites W1992436126 @default.
- W2788974715 cites W2006864699 @default.
- W2788974715 cites W2012617920 @default.
- W2788974715 cites W2023224043 @default.
- W2788974715 cites W2033766616 @default.
- W2788974715 cites W2034557185 @default.
- W2788974715 cites W2035382998 @default.
- W2788974715 cites W2051663103 @default.
- W2788974715 cites W2058213532 @default.
- W2788974715 cites W2061205797 @default.
- W2788974715 cites W2097710423 @default.
- W2788974715 cites W2098597971 @default.
- W2788974715 cites W2102277656 @default.
- W2788974715 cites W2107277218 @default.
- W2788974715 cites W2116631077 @default.
- W2788974715 cites W2131108919 @default.
- W2788974715 cites W2143859712 @default.
- W2788974715 cites W2145383176 @default.
- W2788974715 cites W2147738794 @default.
- W2788974715 cites W2155360759 @default.
- W2788974715 cites W2159513571 @default.
- W2788974715 cites W2168727725 @default.
- W2788974715 cites W2169555946 @default.
- W2788974715 cites W2340109429 @default.
- W2788974715 cites W2418210614 @default.
- W2788974715 cites W2475619206 @default.
- W2788974715 cites W2511030845 @default.
- W2788974715 cites W2531370937 @default.
- W2788974715 cites W2573141618 @default.
- W2788974715 cites W2573412640 @default.
- W2788974715 cites W2730604958 @default.
- W2788974715 cites W746807024 @default.
- W2788974715 doi "https://doi.org/10.1016/j.scienta.2018.01.054" @default.
- W2788974715 hasPublicationYear "2018" @default.
- W2788974715 type Work @default.
- W2788974715 sameAs 2788974715 @default.
- W2788974715 citedByCount "4" @default.
- W2788974715 countsByYear W27889747152022 @default.
- W2788974715 countsByYear W27889747152023 @default.
- W2788974715 crossrefType "journal-article" @default.
- W2788974715 hasAuthorship W2788974715A5018863416 @default.
- W2788974715 hasAuthorship W2788974715A5025649021 @default.
- W2788974715 hasAuthorship W2788974715A5041416953 @default.
- W2788974715 hasAuthorship W2788974715A5047191501 @default.
- W2788974715 hasAuthorship W2788974715A5066886328 @default.
- W2788974715 hasAuthorship W2788974715A5069602593 @default.
- W2788974715 hasAuthorship W2788974715A5083275305 @default.
- W2788974715 hasConcept C104317684 @default.
- W2788974715 hasConcept C116834253 @default.
- W2788974715 hasConcept C150194340 @default.
- W2788974715 hasConcept C189482833 @default.
- W2788974715 hasConcept C196436469 @default.
- W2788974715 hasConcept C54355233 @default.
- W2788974715 hasConcept C59822182 @default.
- W2788974715 hasConcept C86803240 @default.
- W2788974715 hasConceptScore W2788974715C104317684 @default.
- W2788974715 hasConceptScore W2788974715C116834253 @default.
- W2788974715 hasConceptScore W2788974715C150194340 @default.
- W2788974715 hasConceptScore W2788974715C189482833 @default.
- W2788974715 hasConceptScore W2788974715C196436469 @default.
- W2788974715 hasConceptScore W2788974715C54355233 @default.
- W2788974715 hasConceptScore W2788974715C59822182 @default.
- W2788974715 hasConceptScore W2788974715C86803240 @default.
- W2788974715 hasLocation W27889747151 @default.
- W2788974715 hasOpenAccess W2788974715 @default.
- W2788974715 hasPrimaryLocation W27889747151 @default.
- W2788974715 hasRelatedWork W1555022190 @default.
- W2788974715 hasRelatedWork W2002128513 @default.
- W2788974715 hasRelatedWork W2007083730 @default.
- W2788974715 hasRelatedWork W2015887847 @default.
- W2788974715 hasRelatedWork W2078782861 @default.
- W2788974715 hasRelatedWork W2103310157 @default.
- W2788974715 hasRelatedWork W2148374741 @default.
- W2788974715 hasRelatedWork W2171277769 @default.
- W2788974715 hasRelatedWork W3214797750 @default.
- W2788974715 hasRelatedWork W2092874662 @default.
- W2788974715 hasVolume "233" @default.