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- W2789106318 abstract "In vitro culture of Plasmodium vivax liver stages underlies key understandings of the fundamental biology of this parasite, particularly the latent, hyponozoite stage, toward drug and vaccine development. Here, we report systematic production of Plasmodium vivax sporozoites in colonized Anopheles darlingi mosquitoes in the Peruvian Amazon. Human subject-derived P. vivax-infected blood was fed to Anopheles darlingi females using standard membrane feedings assays. Optimizing A. darlingi infection and sporozoite production included replacement of infected patient donor serum with naïve donor serum, comparing anticoagulants in processing blood samples, and addition of penicillin-streptomycin and ATP to infectious blood meals. Replacement of donor serum by naïve serum in the P. vivax donor blood increased oocysts in the mosquito midgut, and heparin, as anticoagulant, was associated with the highest sporozoite yields. Maintaining blood-fed mosquitoes on penicillin-streptomycin in sugar significantly extended mosquito survival which enabled greater sporozoite yield. In this study, we have shown that a robust P. vivax sporozoite production is feasible in a malaria-endemic setting where infected subjects and a stable A. darlingi colony are brought together, with optimized laboratory conditions." @default.
- W2789106318 created "2018-03-06" @default.
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- W2789106318 date "2018-02-21" @default.
- W2789106318 modified "2023-10-14" @default.
- W2789106318 title "Continuous Supply of <i>Plasmodium vivax</i> Sporozoites from Colonized <i>Anopheles darlingi</i> in the Peruvian Amazon" @default.
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- W2789106318 doi "https://doi.org/10.1021/acsinfecdis.7b00195" @default.
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