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• W2789231318 endingPage "3702" @default.
• W2789231318 startingPage "3697" @default.
• W2789231318 abstract "Polymers are commonly used materials for microfluidic chip fabrication, because they are standardized in fabrication and low in cost. However, most polymeric materials that are readily fabricated on the industrial scale are hydrophobic, which is inconvenient for the injection and flow of the aqueous solution, resulting in poor analytical performance for biochemical assays. In this work, we present a straightforward and ultrastable surface modification process for polymeric chips. A one-step modification by using norepinephrine bitartrate monohydrate as a modification reagent is completed at room temperature. The hydrophilicity of the polymeric surfaces increases dramatically. Surface modification is stable for at least 2.5 years, allowing for autoinjection of aqueous solution into the channels. The chips are applied in the immunoassay of alpha-fetoprotein (AFP). The low nonspecific adsorption after modification results in significantly decreased background noise, optimized signal-to-noise ratios (SNR), and dramatically enhanced reproducibility of the immunoassay. Thirty clinical human serum samples are analyzed; these results strongly correlated with the values obtained using commercial test kits. We anticipate that this surface modification method can be used for immunoassay devices in analytical and biosensing technology." @default.
• W2789231318 created "2018-03-06" @default.
• W2789231318 creator A5015569611 @default.
• W2789231318 creator A5051065216 @default.
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• W2789231318 date "2018-02-26" @default.
• W2789231318 modified "2023-10-16" @default.
• W2789231318 title "Straightforward and Ultrastable Surface Modification of Microfluidic Chips with Norepinephrine Bitartrate Improves Performance in Immunoassays" @default.
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• W2789231318 doi "https://doi.org/10.1021/acs.analchem.7b05186" @default.
• W2789231318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29478312" @default.
• W2789231318 hasPublicationYear "2018" @default.
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