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• W2789288460 startingPage "46" @default.
• W2789288460 abstract "In this work, a new strategy of dual-reaction triggered sensitivity amplification for ultrasensitive electrochemical detection of matrix metalloproteinase-7 (MMP-7) was developed. The sensitivity of amperometric biosensor relies on the current signal differences (ΔI) caused by per unit concentration target. Benefited from dual-reaction catalytic activities of Pd nanoparticles, dual catalytic reactions were implemented in the biosensor to amplify the ΔI: (1) Fenton-like reaction was triggered by the probes to degrade redox species methylene blue; (2) catalytic precipitation reaction was followed subsequently to generate insoluble precipitation by 4-chloro-1-naphthol oxidation. Dual-enhancement of ΔI triggered by Pd nanoparticle-based catalytic probes significantly improved the detection performance of the biosensor. The peptide-cleavage based biosensor integrated Pd nanoparticle-based catalytic probes with reduced graphene oxide-Au/methylene blue-sodium alginate hydrogel (Au-rGO/MB-SA) nanocomposites substrate for ultrasensitive detection of MMP-7. Under optimal conditions, the proposed biosensor exhibited a wide linear range from 10 fg mL−1 to 10 ng mL−1 with an ultralow detection limit of 3.1 fg mL−1. This strategy successfully combines the multiple catalytic reactions triggered by nanomaterials with peptide-cleavage pattern in electrochemical biosensor, providing a promising method for detection of other proteases." @default.
• W2789288460 created "2018-03-29" @default.
• W2789288460 creator A5004739436 @default.
• W2789288460 creator A5082251048 @default.
• W2789288460 date "2018-06-01" @default.
• W2789288460 modified "2023-10-10" @default.
• W2789288460 title "Dual-reaction triggered sensitivity amplification for ultrasensitive peptide-cleavage based electrochemical detection of matrix metalloproteinase-7" @default.
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• W2789288460 doi "https://doi.org/10.1016/j.bios.2018.02.045" @default.
• W2789288460 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29499558" @default.
• W2789288460 hasPublicationYear "2018" @default.
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