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• W2789585890 abstract "In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material. The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m2/g. After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism. Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 μg/μL, and the detection limit of glycans could be down to 1 ng/μL with 21 N-linked glycans identified. When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified. From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon. In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan." @default.
• W2789585890 created "2018-03-29" @default.
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• W2789585890 date "2018-03-16" @default.
• W2789585890 modified "2023-10-18" @default.
• W2789585890 title "Highly Porous Metal-Free Graphitic Carbon Derived from Metal–Organic Framework for Profiling of N-Linked Glycans" @default.
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• W2789585890 doi "https://doi.org/10.1021/acsami.8b02423" @default.
• W2789585890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29546983" @default.
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