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• W2789649543 abstract "Epigenetic factors such as histone modifications play integral roles in plant development and stress response, yet their implications in algae remain poorly understood. In the industrial oleaginous microalgae Nannochloropsis spp., the lack of an efficient methodology for chromatin immunoprecipitation (ChIP), which determines the specific genomic location of various histone modifications, has hindered probing the epigenetic basis of their photosynthetic carbon conversion and storage as oil. Here, a detailed ChIP protocol was developed for Nannochloropsis oceanica, which represents a reliable approach for the analysis of histone modifications, chromatin state, and transcription factor-binding sites at the epigenetic level. Using ChIP-qPCR, genes related to photosynthetic carbon fixation in this microalga were systematically assessed. Furthermore, a ChIP-Seq protocol was established and optimized, which generated a genome-wide profile of histone modification events, using histone mark H3K9Ac as an example. These results are the first step for appreciation of the chromatin landscape in industrial oleaginous microalgae and for epigenetics-based microalgal feedstock development." @default.
• W2789649543 created "2018-03-29" @default.
• W2789649543 creator A5018135780 @default.
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• W2789649543 date "2018-03-13" @default.
• W2789649543 modified "2023-10-14" @default.
• W2789649543 title "Optimized methods of chromatin immunoprecipitation for profiling histone modifications in industrial microalgae <i>Nannochloropsis</i> spp." @default.
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• W2789649543 doi "https://doi.org/10.1111/jpy.12623" @default.
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