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- W2789658510 abstract "Abstract Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a−f and nontypeable; n = 258) and isogenic capsule transformants (types a−d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a−f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae." @default.
- W2789658510 created "2018-03-29" @default.
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- W2789658510 date "2018-03-01" @default.
- W2789658510 modified "2023-10-12" @default.
- W2789658510 title "Capsule Typing of<i>Haemophilus influenzae</i>by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry1" @default.
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- W2789658510 doi "https://doi.org/10.3201/eid2403.170459" @default.
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