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• W2789668001 abstract "DNA and histone methylation coregulate heterochromatin formation and gene silencing in animals and plants. To identify factors involved in maintaining gene silencing, we conducted a forward genetic screen for mutants that release the silenced transgene Pro35S::NEOMYCIN PHOSPHOTRANSFERASE II in the transgenic Arabidopsis (Arabidopsis thaliana) line L119 We identified MAT4/SAMS3/MTO3/AT3G17390, which encodes methionine (Met) adenosyltransferase 4 (MAT4)/S-adenosyl-Met synthetase 3 that catalyzes the synthesis of S-adenosyl-Met (SAM) in the one-carbon metabolism cycle. mat4 mostly decreases CHG and CHH DNA methylation and histone H3K9me2 and reactivates certain silenced transposons. The exogenous addition of SAM partially rescues the epigenetic defects of mat4 SAM content and DNA methylation were reduced more in mat4 than in three other mat mutants. MAT4 knockout mutations generated by CRISPR/Cas9 were lethal, indicating that MAT4 is an essential gene in Arabidopsis. MAT1, 2, and 4 proteins exhibited nearly equal activity in an in vitro assay, whereas MAT3 exhibited higher activity. The native MAT4 promoter driving MAT1, 2, and 3 cDNA complemented the mat4 mutant. However, most mat4 transgenic lines carrying native MAT1, 2, and 3 promoters driving MAT4 cDNA did not complement the mat4 mutant because of their lower expression in seedlings. Genetic analyses indicated that the mat1mat4 double mutant is dwarfed and the mat2mat4 double mutant was nonviable, while mat1mat2 showed normal growth and fertility. These results indicate that MAT4 plays a predominant role in SAM production, plant growth, and development. Our findings provide direct evidence of the cooperative actions between metabolism and epigenetic regulation." @default.
• W2789668001 created "2018-03-29" @default.
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• W2789668001 date "2018-03-23" @default.
• W2789668001 modified "2023-10-16" @default.
• W2789668001 title "METHIONINE ADENOSYLTRANSFERASE4 Mediates DNA and Histone Methylation" @default.
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• W2789668001 cites W1527275481 @default.
• W2789668001 cites W1562187797 @default.
• W2789668001 cites W1817033456 @default.
• W2789668001 cites W1838617752 @default.
• W2789668001 cites W1937054661 @default.
• W2789668001 cites W1967655525 @default.
• W2789668001 cites W1968466047 @default.
• W2789668001 cites W1975787171 @default.
• W2789668001 cites W1977461843 @default.
• W2789668001 cites W1981669094 @default.
• W2789668001 cites W1984251741 @default.
• W2789668001 cites W1999644599 @default.
• W2789668001 cites W2011121716 @default.
• W2789668001 cites W2016813268 @default.
• W2789668001 cites W2019525237 @default.
• W2789668001 cites W2020173141 @default.
• W2789668001 cites W2026343134 @default.
• W2789668001 cites W2027713539 @default.
• W2789668001 cites W2030132680 @default.
• W2789668001 cites W2030506347 @default.
• W2789668001 cites W2037258151 @default.
• W2789668001 cites W2041680957 @default.
• W2789668001 cites W2041707026 @default.
• W2789668001 cites W2043405255 @default.
• W2789668001 cites W2047011611 @default.
• W2789668001 cites W2050669688 @default.
• W2789668001 cites W2056077438 @default.
• W2789668001 cites W2058040756 @default.
• W2789668001 cites W2067254288 @default.
• W2789668001 cites W2095750709 @default.
• W2789668001 cites W2100999894 @default.
• W2789668001 cites W2106035323 @default.
• W2789668001 cites W2107314112 @default.
• W2789668001 cites W2110818034 @default.
• W2789668001 cites W2112903461 @default.
• W2789668001 cites W2113643663 @default.
• W2789668001 cites W2114104545 @default.
• W2789668001 cites W2116122104 @default.
• W2789668001 cites W2118753423 @default.
• W2789668001 cites W2119585159 @default.
• W2789668001 cites W2121304801 @default.
• W2789668001 cites W2122818883 @default.
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• W2789668001 cites W2126307925 @default.
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• W2789668001 cites W2131374955 @default.
• W2789668001 cites W2133622062 @default.
• W2789668001 cites W2137981728 @default.
• W2789668001 cites W2139460087 @default.
• W2789668001 cites W2144078257 @default.
• W2789668001 cites W2146543824 @default.
• W2789668001 cites W2151439672 @default.
• W2789668001 cites W2152173510 @default.
• W2789668001 cites W2158335648 @default.
• W2789668001 cites W2158804744 @default.
• W2789668001 cites W2162648640 @default.
• W2789668001 cites W2163851955 @default.
• W2789668001 cites W2165290732 @default.
• W2789668001 cites W2166404742 @default.
• W2789668001 cites W2171644358 @default.
• W2789668001 cites W2192908784 @default.
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• W2789668001 doi "https://doi.org/10.1104/pp.18.00183" @default.
• W2789668001 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6001336" @default.
• W2789668001 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29572390" @default.
• W2789668001 hasPublicationYear "2018" @default.
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