Matches in SemOpenAlex for { <https://semopenalex.org/work/W2789873873> ?p ?o ?g. }
- W2789873873 endingPage "114" @default.
- W2789873873 startingPage "109" @default.
- W2789873873 abstract "The Fe(II)/2-oxoglutarate-dependent dioxygenases (2-OGDs) catalyze the oxidation of substrates ranging from small molecules to large biomolecules with concomitant oxidation of co-substrate (2-oxoglutarate) into succinate. In the present study, we reported a coupled colorimetric assay that can be generally applied to measure the activities of all members of 2-OGDs family. Succinyl-CoA synthetase is employed as the coupling enzyme to transform succinate produced from 2-OGDs catalysis to form succinyl-CoA with concomitant hydrolysis of ATP to form ADP and orthophosphate. Orthophosphate can be quantitated by reacting it with molybdic acid forming a blue pigment. As a proof of concept, kinetic parameters of ectoine hydroxylase obtained using this method are compared to a traditional time- and labor-consuming HPLC based method. As 2-OGDs family enzymes are important drug targets due to their impressive versatility in catalyzing numerous oxidative reactions that are still very challenging using synthetic chemistry, colorimetric method detailed in the manuscript has the potential to enable the practice of high throughput drug screening for 2-OGDs." @default.
- W2789873873 created "2018-03-29" @default.
- W2789873873 creator A5005075235 @default.
- W2789873873 creator A5039182454 @default.
- W2789873873 creator A5049726012 @default.
- W2789873873 creator A5055428913 @default.
- W2789873873 creator A5066342455 @default.
- W2789873873 date "2018-05-01" @default.
- W2789873873 modified "2023-10-18" @default.
- W2789873873 title "Developing a colorimetric assay for Fe(II)/2-oxoglutarate-dependent dioxygenase" @default.
- W2789873873 cites W1483811243 @default.
- W2789873873 cites W1602845909 @default.
- W2789873873 cites W1654652909 @default.
- W2789873873 cites W1762414595 @default.
- W2789873873 cites W1792646340 @default.
- W2789873873 cites W1843159808 @default.
- W2789873873 cites W1922028241 @default.
- W2789873873 cites W1972021276 @default.
- W2789873873 cites W1974263523 @default.
- W2789873873 cites W1981777803 @default.
- W2789873873 cites W1983812869 @default.
- W2789873873 cites W1987517030 @default.
- W2789873873 cites W1988782706 @default.
- W2789873873 cites W1997814329 @default.
- W2789873873 cites W2001420616 @default.
- W2789873873 cites W2005113813 @default.
- W2789873873 cites W2005750198 @default.
- W2789873873 cites W2006575088 @default.
- W2789873873 cites W2009723401 @default.
- W2789873873 cites W2010060668 @default.
- W2789873873 cites W2013104295 @default.
- W2789873873 cites W2025443699 @default.
- W2789873873 cites W2026625413 @default.
- W2789873873 cites W2030884466 @default.
- W2789873873 cites W2039347752 @default.
- W2789873873 cites W2054643318 @default.
- W2789873873 cites W2061657754 @default.
- W2789873873 cites W2064171176 @default.
- W2789873873 cites W2066263439 @default.
- W2789873873 cites W2069351864 @default.
- W2789873873 cites W2086342381 @default.
- W2789873873 cites W2092762055 @default.
- W2789873873 cites W2116571550 @default.
- W2789873873 cites W2128120865 @default.
- W2789873873 cites W2138992920 @default.
- W2789873873 cites W2145023430 @default.
- W2789873873 cites W2171490882 @default.
- W2789873873 cites W2186840966 @default.
- W2789873873 cites W2265897459 @default.
- W2789873873 cites W2304414816 @default.
- W2789873873 cites W2320117176 @default.
- W2789873873 cites W2559814159 @default.
- W2789873873 cites W4249433407 @default.
- W2789873873 doi "https://doi.org/10.1016/j.ab.2018.02.013" @default.
- W2789873873 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29499175" @default.
- W2789873873 hasPublicationYear "2018" @default.
- W2789873873 type Work @default.
- W2789873873 sameAs 2789873873 @default.
- W2789873873 citedByCount "4" @default.
- W2789873873 countsByYear W27898738732019 @default.
- W2789873873 countsByYear W27898738732020 @default.
- W2789873873 countsByYear W27898738732023 @default.
- W2789873873 crossrefType "journal-article" @default.
- W2789873873 hasAuthorship W2789873873A5005075235 @default.
- W2789873873 hasAuthorship W2789873873A5039182454 @default.
- W2789873873 hasAuthorship W2789873873A5049726012 @default.
- W2789873873 hasAuthorship W2789873873A5055428913 @default.
- W2789873873 hasAuthorship W2789873873A5066342455 @default.
- W2789873873 hasConcept C111368507 @default.
- W2789873873 hasConcept C127313418 @default.
- W2789873873 hasConcept C161790260 @default.
- W2789873873 hasConcept C178790620 @default.
- W2789873873 hasConcept C181199279 @default.
- W2789873873 hasConcept C185592680 @default.
- W2789873873 hasConcept C21951064 @default.
- W2789873873 hasConcept C2776834422 @default.
- W2789873873 hasConcept C2777289219 @default.
- W2789873873 hasConcept C32909587 @default.
- W2789873873 hasConcept C49853544 @default.
- W2789873873 hasConcept C51323132 @default.
- W2789873873 hasConcept C55493867 @default.
- W2789873873 hasConcept C94412978 @default.
- W2789873873 hasConceptScore W2789873873C111368507 @default.
- W2789873873 hasConceptScore W2789873873C127313418 @default.
- W2789873873 hasConceptScore W2789873873C161790260 @default.
- W2789873873 hasConceptScore W2789873873C178790620 @default.
- W2789873873 hasConceptScore W2789873873C181199279 @default.
- W2789873873 hasConceptScore W2789873873C185592680 @default.
- W2789873873 hasConceptScore W2789873873C21951064 @default.
- W2789873873 hasConceptScore W2789873873C2776834422 @default.
- W2789873873 hasConceptScore W2789873873C2777289219 @default.
- W2789873873 hasConceptScore W2789873873C32909587 @default.
- W2789873873 hasConceptScore W2789873873C49853544 @default.
- W2789873873 hasConceptScore W2789873873C51323132 @default.
- W2789873873 hasConceptScore W2789873873C55493867 @default.
- W2789873873 hasConceptScore W2789873873C94412978 @default.
- W2789873873 hasFunder F4320321001 @default.
- W2789873873 hasLocation W27898738731 @default.