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- W2790103923 abstract "Coumarin derivatives are well known fluorescence reporters for investigating biological systems due to their strong micro-environment sensitivity. Despite having wide range of environment sensitive fluorescence probes, the potential of 6,7-dimethoxy-coumarin has not been studied extensively so far. With a perspective of its use in protein studies, namely using the unnatural amino acid technology or as a substrate for hydrolase enzymes, we study acetyloxymethyl-6,7-dimethoxycoumarin (Ac-DMC). We investigate the photophysics and hydration dynamics of this dye in aerosol-OT (AOT) reverse micelles at various water contents using the time dependent fluorescence shift (TDFS) method. The TDFS response in AOT reverse micelles from water/surfactant ratio of 0 to 20 confirms its sensitivity towards the hydration and mobility of its microenvironment. Moreover, we show that the fluorophore can be efficiently quenched by halide ions. Hence, we conclude that the 6,7-dimethoxy-methylcoumarin fluorophore is useful for studying hydration parameters in biologically relevant systems." @default.
- W2790103923 created "2018-03-29" @default.
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- W2790103923 date "2018-02-12" @default.
- W2790103923 modified "2023-10-16" @default.
- W2790103923 title "6,7-dimethoxy-coumarin as a probe of hydration dynamics in biologically relevant systems" @default.
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- W2790103923 doi "https://doi.org/10.1088/2050-6120/aaaa05" @default.
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