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• W2790141330 abstract "Adenosine is an endogenous purine nucleoside that acts in all living systems as a homeostatic network regulator through many pathways, which are adenosine receptor (AR)-dependent and -independent. From a metabolic point of view, adenosine deaminase (ADA) is an essential protein in the regulation of the total intracellular and extracellular adenosine in a tissue. In addition to its cytosolic localization, ADA is also expressed as an ecto-enzyme on the surface of different cells. Dipeptidyl peptidase IV (CD26) and some ARs act as binding proteins for extracellular ADA in humans. Since CD26 and ARs interact with ADA at opposite sites, we have investigated if ADA can function as a cell-to-cell communication molecule by bridging the anchoring molecules CD26 and A2AR present on the surfaces of the interacting cells. By combining site-directed mutagenesis of ADA amino acids involved in binding to A2AR and a modification of the bioluminescence resonance energy transfer (BRET) technique that allows detection of interactions between two proteins expressed in different cell populations with low steric hindrance (NanoBRET), we show direct evidence of the specific formation of trimeric complexes CD26-ADA-A2AR involving two cells. By dynamic mass redistribution assays and ligand binding experiments, we also demonstrate that A2AR-NanoLuc fusion proteins are functional. The existence of this ternary complex is in good agreement with the hypothesis that ADA could bridge T-cells (expressing CD26) and dendritic cells (expressing A2AR). This is a new metabolic function for ecto-ADA that, being a single chain protein, it has been considered as an example of moonlighting protein, because it performs more than one functional role (as a catalyst, a costimulator, an allosteric modulator and a cell-to-cell connector) without partitioning these functions in different subunits." @default.
• W2790141330 created "2018-03-29" @default.
• W2790141330 creator A5013842852 @default.
• W2790141330 creator A5026462548 @default.
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• W2790141330 creator A5056422298 @default.
• W2790141330 creator A5069338728 @default.
• W2790141330 creator A5073558772 @default.
• W2790141330 date "2018-02-15" @default.
• W2790141330 modified "2023-09-27" @default.
• W2790141330 title "Molecular Evidence of Adenosine Deaminase Linking Adenosine A2A Receptor and CD26 Proteins" @default.
• W2790141330 cites W1480108271 @default.
• W2790141330 cites W1484296597 @default.
• W2790141330 cites W1490075410 @default.
• W2790141330 cites W1495150305 @default.
• W2790141330 cites W1522255132 @default.
• W2790141330 cites W154499290 @default.
• W2790141330 cites W1565181678 @default.
• W2790141330 cites W1576503935 @default.
• W2790141330 cites W1581333439 @default.
• W2790141330 cites W1595377541 @default.
• W2790141330 cites W1599893459 @default.
• W2790141330 cites W1780426348 @default.
• W2790141330 cites W1783256508 @default.
• W2790141330 cites W1819331143 @default.
• W2790141330 cites W1870664662 @default.
• W2790141330 cites W1885520199 @default.
• W2790141330 cites W1894872128 @default.
• W2790141330 cites W1904592173 @default.
• W2790141330 cites W1926839736 @default.
• W2790141330 cites W1937764641 @default.
• W2790141330 cites W1941245750 @default.
• W2790141330 cites W1958779778 @default.
• W2790141330 cites W1964365720 @default.
• W2790141330 cites W1964421631 @default.
• W2790141330 cites W1964534311 @default.
• W2790141330 cites W1968777484 @default.
• W2790141330 cites W1970188140 @default.
• W2790141330 cites W1973799342 @default.
• W2790141330 cites W1975391500 @default.
• W2790141330 cites W1975740938 @default.
• W2790141330 cites W1979693948 @default.
• W2790141330 cites W1980247527 @default.
• W2790141330 cites W1980962873 @default.
• W2790141330 cites W1981464756 @default.
• W2790141330 cites W1981768599 @default.
• W2790141330 cites W1982540896 @default.
• W2790141330 cites W1985598247 @default.
• W2790141330 cites W1986261444 @default.
• W2790141330 cites W1987788453 @default.
• W2790141330 cites W1988887233 @default.
• W2790141330 cites W1989197835 @default.
• W2790141330 cites W1990607669 @default.
• W2790141330 cites W1991504299 @default.
• W2790141330 cites W1995144601 @default.
• W2790141330 cites W1995895217 @default.
• W2790141330 cites W1996774458 @default.
• W2790141330 cites W2003687681 @default.
• W2790141330 cites W2005722172 @default.
• W2790141330 cites W2006084592 @default.
• W2790141330 cites W2006297395 @default.
• W2790141330 cites W2006299048 @default.
• W2790141330 cites W2009846592 @default.
• W2790141330 cites W2012412668 @default.
• W2790141330 cites W2014365582 @default.
• W2790141330 cites W2014854059 @default.
• W2790141330 cites W2016950608 @default.
• W2790141330 cites W2020051426 @default.
• W2790141330 cites W2021589497 @default.
• W2790141330 cites W2021614849 @default.
• W2790141330 cites W2023530341 @default.
• W2790141330 cites W2025077799 @default.
• W2790141330 cites W2028849170 @default.
• W2790141330 cites W2029391645 @default.
• W2790141330 cites W2038201483 @default.
• W2790141330 cites W2038662217 @default.
• W2790141330 cites W2038848067 @default.
• W2790141330 cites W2039399096 @default.
• W2790141330 cites W2039872108 @default.
• W2790141330 cites W2040164212 @default.
• W2790141330 cites W2046507356 @default.
• W2790141330 cites W2046849514 @default.
• W2790141330 cites W2049561707 @default.
• W2790141330 cites W2053376664 @default.
• W2790141330 cites W2056222368 @default.
• W2790141330 cites W2059804447 @default.
• W2790141330 cites W2066684868 @default.
• W2790141330 cites W2067653726 @default.
• W2790141330 cites W2069523155 @default.
• W2790141330 cites W2069790342 @default.
• W2790141330 cites W2071601558 @default.
• W2790141330 cites W2073477111 @default.
• W2790141330 cites W2074146366 @default.
• W2790141330 cites W2078529338 @default.
• W2790141330 cites W2078911982 @default.
• W2790141330 cites W2079236755 @default.
• W2790141330 cites W2081100883 @default.
• W2790141330 cites W2083075330 @default.

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