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- W2790192404 abstract "Despite significant progress in polarized light microscopy, measuring the three dimensional anisotropy is still an important challenge in the analysis of anisotropic materials. In birefringent samples, traditional imaging methods don't reveal the 3D orientation (inclination and azimuth) of the optic axis and the birefringence as an intrinsic material property. In samples that emit polarized fluorescence, it is generally not possible to discern the inclination angle of the aligned fluorophores. To overcome these problems, the Oldenbourg Lab has introduced a new imaging method, the Light Field LC-PolScope, which is a combination of light field imaging and LC-PolScope technology. In our poster, we have used the Light Field LC-PolScope for acquiring images of simple and biological relevant test specimens for developing processing methods to reconstruct the birefringence of juvenile shells of the Atlantic hard clam and the polarized fluorescence of the labeled cell membrane of sea urchin eggs. We established acquisition and processing methods to generate 3-dimensional maps of position and orientation of these optically anisotropic structures, based on polarized light field images and compare results to more traditional imaging methods. Our work represents the first successful attempt to generate such maps at a lateral resolution of 2µm and angular steps of 9° in terms of the azimuth and inclination angles of the optic axis. The maps of clam shell birefringence provide structural insights into the early mineralization during juvenile clam shell development." @default.
- W2790192404 created "2018-03-29" @default.
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- W2790192404 date "2018-02-01" @default.
- W2790192404 modified "2023-09-30" @default.
- W2790192404 title "Light Field LC-PolScope" @default.
- W2790192404 doi "https://doi.org/10.1016/j.bpj.2017.11.2908" @default.
- W2790192404 hasPublicationYear "2018" @default.
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