Matches in SemOpenAlex for { <https://semopenalex.org/work/W2790456375> ?p ?o ?g. }
- W2790456375 endingPage "687" @default.
- W2790456375 startingPage "683" @default.
- W2790456375 abstract "The optimization of engineered metabolic pathways requires careful control over the levels and timing of metabolic enzyme expression. Optogenetic tools are ideal for achieving such precise control, as light can be applied and removed instantly without complex media changes. Here we show that light-controlled transcription can be used to enhance the biosynthesis of valuable products in engineered Saccharomyces cerevisiae. We introduce new optogenetic circuits to shift cells from a light-induced growth phase to a darkness-induced production phase, which allows us to control fermentation with only light. Furthermore, optogenetic control of engineered pathways enables a new mode of bioreactor operation using periodic light pulses to tune enzyme expression during the production phase of fermentation to increase yields. Using these advances, we control the mitochondrial isobutanol pathway to produce up to 8.49 ± 0.31 g l-1 of isobutanol and 2.38 ± 0.06 g l-1 of 2-methyl-1-butanol micro-aerobically from glucose. These results make a compelling case for the application of optogenetics to metabolic engineering for the production of valuable products." @default.
- W2790456375 created "2018-03-29" @default.
- W2790456375 creator A5031697521 @default.
- W2790456375 creator A5040927133 @default.
- W2790456375 creator A5047376321 @default.
- W2790456375 creator A5064574606 @default.
- W2790456375 creator A5071413814 @default.
- W2790456375 creator A5077735957 @default.
- W2790456375 creator A5086331232 @default.
- W2790456375 date "2018-03-01" @default.
- W2790456375 modified "2023-10-18" @default.
- W2790456375 title "Optogenetic regulation of engineered cellular metabolism for microbial chemical production" @default.
- W2790456375 cites W1850569493 @default.
- W2790456375 cites W1971439989 @default.
- W2790456375 cites W1988165722 @default.
- W2790456375 cites W1990060260 @default.
- W2790456375 cites W1993724093 @default.
- W2790456375 cites W1994794118 @default.
- W2790456375 cites W1996629685 @default.
- W2790456375 cites W1997859335 @default.
- W2790456375 cites W2001831734 @default.
- W2790456375 cites W2003885556 @default.
- W2790456375 cites W2005454774 @default.
- W2790456375 cites W2045293869 @default.
- W2790456375 cites W2046624199 @default.
- W2790456375 cites W2063184965 @default.
- W2790456375 cites W2083149221 @default.
- W2790456375 cites W2101700211 @default.
- W2790456375 cites W2105140815 @default.
- W2790456375 cites W2111997766 @default.
- W2790456375 cites W2114712156 @default.
- W2790456375 cites W2119059442 @default.
- W2790456375 cites W2120988439 @default.
- W2790456375 cites W2133071016 @default.
- W2790456375 cites W2139409523 @default.
- W2790456375 cites W2149202606 @default.
- W2790456375 cites W2159958449 @default.
- W2790456375 cites W2165630230 @default.
- W2790456375 cites W2168059482 @default.
- W2790456375 cites W2228834077 @default.
- W2790456375 cites W2277766365 @default.
- W2790456375 cites W2326511145 @default.
- W2790456375 cites W2332181118 @default.
- W2790456375 cites W2397987827 @default.
- W2790456375 cites W2412228714 @default.
- W2790456375 cites W2464884339 @default.
- W2790456375 cites W2470764148 @default.
- W2790456375 cites W2473378339 @default.
- W2790456375 cites W2507618556 @default.
- W2790456375 cites W2564496757 @default.
- W2790456375 cites W2588061211 @default.
- W2790456375 cites W2763611320 @default.
- W2790456375 cites W2951224618 @default.
- W2790456375 cites W4210574564 @default.
- W2790456375 doi "https://doi.org/10.1038/nature26141" @default.
- W2790456375 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5876151" @default.
- W2790456375 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29562237" @default.
- W2790456375 hasPublicationYear "2018" @default.
- W2790456375 type Work @default.
- W2790456375 sameAs 2790456375 @default.
- W2790456375 citedByCount "241" @default.
- W2790456375 countsByYear W27904563752018 @default.
- W2790456375 countsByYear W27904563752019 @default.
- W2790456375 countsByYear W27904563752020 @default.
- W2790456375 countsByYear W27904563752021 @default.
- W2790456375 countsByYear W27904563752022 @default.
- W2790456375 countsByYear W27904563752023 @default.
- W2790456375 crossrefType "journal-article" @default.
- W2790456375 hasAuthorship W2790456375A5031697521 @default.
- W2790456375 hasAuthorship W2790456375A5040927133 @default.
- W2790456375 hasAuthorship W2790456375A5047376321 @default.
- W2790456375 hasAuthorship W2790456375A5064574606 @default.
- W2790456375 hasAuthorship W2790456375A5071413814 @default.
- W2790456375 hasAuthorship W2790456375A5077735957 @default.
- W2790456375 hasAuthorship W2790456375A5086331232 @default.
- W2790456375 hasBestOaLocation W27904563752 @default.
- W2790456375 hasConcept C100544194 @default.
- W2790456375 hasConcept C169760540 @default.
- W2790456375 hasConcept C181199279 @default.
- W2790456375 hasConcept C185592680 @default.
- W2790456375 hasConcept C191908910 @default.
- W2790456375 hasConcept C192989942 @default.
- W2790456375 hasConcept C2777271787 @default.
- W2790456375 hasConcept C2777576037 @default.
- W2790456375 hasConcept C2779222958 @default.
- W2790456375 hasConcept C2781066024 @default.
- W2790456375 hasConcept C50738837 @default.
- W2790456375 hasConcept C55493867 @default.
- W2790456375 hasConcept C6350086 @default.
- W2790456375 hasConcept C70721500 @default.
- W2790456375 hasConcept C86803240 @default.
- W2790456375 hasConcept C95444343 @default.
- W2790456375 hasConceptScore W2790456375C100544194 @default.
- W2790456375 hasConceptScore W2790456375C169760540 @default.
- W2790456375 hasConceptScore W2790456375C181199279 @default.
- W2790456375 hasConceptScore W2790456375C185592680 @default.
- W2790456375 hasConceptScore W2790456375C191908910 @default.
- W2790456375 hasConceptScore W2790456375C192989942 @default.