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• W2790485718 abstract "MicroRNAs (miRNAs), small 22-25 nucleotide non-coding RNAs, play important roles in cellular and tumor biology. However, characterizing miRNA function remains challenging due to an abundance of predicted targets and an experimental bottleneck in identifying biologically relevant direct targets. Here, we developed a novel technique (miFAST) to identify direct miRNA target genes. Using miFAST, we confirmed several previously reported miR-340 target genes and identified five additional novel direct miR-340 targets in melanoma cells. This methodology can also be efficiently applied for the global characterization of miRNA targets. Utilizing miFAST to characterize direct miRNA targetomes will further our understanding of miRNA biology and function." @default.
• W2790485718 created "2018-03-29" @default.
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• W2790485718 date "2018-02-12" @default.
• W2790485718 modified "2023-09-25" @default.
• W2790485718 title "miFAST: A novel and rapid microRNA target capture method" @default.
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• W2790485718 doi "https://doi.org/10.1002/mc.22780" @default.
• W2790485718 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29350431" @default.
• W2790485718 hasPublicationYear "2018" @default.
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