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• W2790556232 abstract "<b>Background: </b>The detection of irregular antibody is a critical issue in the management of red blood cell transfusion according to the Type &amp; Screen (T&amp;S) practice. In order to implement the T&amp;S procedure at our blood bank, we compared three different automated analyzers based on column agglutination technique (CAT) or solid phase red cell adherence assay (SPACA) methods. <b>Methods: </b>Pre-transfusion antibody screening was performed in 986 patients candidate to elective surgery at low risk for red blood cell transfusion. We tested the following kits: the three-cell panel micro-CAT system ID-DiaCell I-II-III (DiaMed), the four-cell panel solid-phase system Capture-R Ready Screen-4 (Immucor), and the four-cell panel micro-CAT system Serascan Diana-4 (Grifols). Positive results were further investigated using corresponding identification panels, and discrepant results were investigated with all the antibody identification systems. <b>Results:</b> Among 986 samples, we observed 967 concordant negative results (98.1%), 8 concordant positive results (0.8% of cases), and 11 discrepant results (1.1%). Among discrepant samples, an alloantibody could been identified in two patents (anti-M, detected by Serascan Diana-4 and ID-DiaCell I, II, III; anti-Kp^a, detected by Capture-R Ready Screen-4 and Serascan Diana-4). <b>Conclusion: </b>Among the evaluated technologies<b>, </b>the four-cell panel micro-CAT system displayed the highest sensitivity and specificity with an optimal negative predictive value. These features might be relevant to the routine implementation of the T&amp;S transfusion strategy." @default.
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• W2790556232 date "2018-01-01" @default.
• W2790556232 modified "2023-10-02" @default.
• W2790556232 title "Red Cell Alloantibody Screening: Comparative Analysis of Three Different Technologies" @default.
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• W2790556232 doi "https://doi.org/10.1159/000484570" @default.
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