FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2790598255> ?p ?o ?g. }

• W2790598255 abstract "PrtA is the major secreted metalloprotease of Serratia marcescens Previous reports implicate PrtA in the pathogenic capacity of this bacterium. PrtA is also clinically used as a potent analgesic and anti-inflammatory drug, and its catalytic properties attract industrial interest. Comparatively, there is scarce knowledge about the mechanisms that physiologically govern PrtA expression in Serratia In this work, we demonstrate that PrtA production is derepressed when the bacterial growth temperature decreases from 37°C to 30°C. We show that this thermoregulation occurs at the transcriptional level. We determined that upstream of prtA, there is a conserved motif that is directly recognized by the CpxR transcriptional regulator. This feature is found along Serratia strains irrespective of their isolation source, suggesting an evolutionary conservation of CpxR-dependent regulation of PrtA expression. We found that in S. marcescens, the CpxAR system is more active at 37°C than at 30°C. In good agreement with these results, in a cpxR mutant background, prtA is derepressed at 37°C, while overexpression of the NlpE lipoprotein, a well-known CpxAR-inducing condition, inhibits PrtA expression, suggesting that the levels of the activated form of CpxR are increased at 37°C over those at 30°C. In addition, we establish that PrtA is involved in the ability of S. marcescens to develop biofilm. In accordance, CpxR influences the biofilm phenotype only when bacteria are grown at 37°C. In sum, our findings shed light on regulatory mechanisms that fine-tune PrtA expression and reveal a novel role for PrtA in the lifestyle of S. marcescensIMPORTANCE We demonstrate that S. marcescens metalloprotease PrtA expression is transcriptionally thermoregulated. While strongly activated below 30°C, its expression is downregulated at 37°C. We found that in S. marcescens, the CpxAR signal transduction system, which responds to envelope stress and bacterial surface adhesion, is activated at 37°C and able to downregulate PrtA expression by direct interaction of CpxR with a binding motif located upstream of the prtA gene. Moreover, we reveal that PrtA expression favors the ability of S. marcescens to develop biofilm, irrespective of the bacterial growth temperature. In this context, thermoregulation along with a highly conserved CpxR-dependent modulation mechanism gives clues about the relevance of PrtA as a factor implicated in the persistence of S. marcescens on abiotic surfaces and in bacterial host colonization capacity." @default.
• W2790598255 created "2018-03-29" @default.
• W2790598255 creator A5035588345 @default.
• W2790598255 creator A5040662411 @default.
• W2790598255 creator A5040668425 @default.
• W2790598255 creator A5042102031 @default.
• W2790598255 creator A5053252309 @default.
• W2790598255 date "2018-04-15" @default.
• W2790598255 modified "2023-10-16" @default.
• W2790598255 title "CpxR-Dependent Thermoregulation of Serratia marcescens PrtA Metalloprotease Expression and Its Contribution to Bacterial Biofilm Formation" @default.
• W2790598255 cites W1495142741 @default.
• W2790598255 cites W165860796 @default.
• W2790598255 cites W1751563601 @default.
• W2790598255 cites W1817288881 @default.
• W2790598255 cites W1818048824 @default.
• W2790598255 cites W1822842365 @default.
• W2790598255 cites W1835868 @default.
• W2790598255 cites W1971806607 @default.
• W2790598255 cites W1976095793 @default.
• W2790598255 cites W1977389772 @default.
• W2790598255 cites W1984974565 @default.
• W2790598255 cites W1987061077 @default.
• W2790598255 cites W1989183414 @default.
• W2790598255 cites W2004867814 @default.
• W2790598255 cites W2008768202 @default.
• W2790598255 cites W2009376936 @default.
• W2790598255 cites W2010705215 @default.
• W2790598255 cites W2016549645 @default.
• W2790598255 cites W2018632977 @default.
• W2790598255 cites W2020720083 @default.
• W2790598255 cites W2024673940 @default.
• W2790598255 cites W2029316600 @default.
• W2790598255 cites W2032092088 @default.
• W2790598255 cites W2036917159 @default.
• W2790598255 cites W2039224522 @default.
• W2790598255 cites W2043716056 @default.
• W2790598255 cites W2048329119 @default.
• W2790598255 cites W2050857190 @default.
• W2790598255 cites W2051355114 @default.
• W2790598255 cites W2051453478 @default.
• W2790598255 cites W2057463863 @default.
• W2790598255 cites W2061126505 @default.
• W2790598255 cites W2062221023 @default.
• W2790598255 cites W2064772802 @default.
• W2790598255 cites W2071942350 @default.
• W2790598255 cites W2084622748 @default.
• W2790598255 cites W2088210549 @default.
• W2790598255 cites W2097191787 @default.
• W2790598255 cites W2097899215 @default.
• W2790598255 cites W2098464894 @default.
• W2790598255 cites W2105324903 @default.
• W2790598255 cites W2111194455 @default.
• W2790598255 cites W2114294973 @default.
• W2790598255 cites W2114447433 @default.
• W2790598255 cites W2114898983 @default.
• W2790598255 cites W2116021469 @default.
• W2790598255 cites W2117320189 @default.
• W2790598255 cites W2120990306 @default.
• W2790598255 cites W2123712967 @default.
• W2790598255 cites W2132296494 @default.
• W2790598255 cites W2133760875 @default.
• W2790598255 cites W2134696956 @default.
• W2790598255 cites W2137371074 @default.
• W2790598255 cites W2138931605 @default.
• W2790598255 cites W2140952049 @default.
• W2790598255 cites W2142146745 @default.
• W2790598255 cites W2142761155 @default.
• W2790598255 cites W2155581972 @default.
• W2790598255 cites W2157211922 @default.
• W2790598255 cites W2157642203 @default.
• W2790598255 cites W2160309830 @default.
• W2790598255 cites W2166759385 @default.
• W2790598255 cites W2173928087 @default.
• W2790598255 cites W2187216847 @default.
• W2790598255 cites W2205963623 @default.
• W2790598255 cites W2319901721 @default.
• W2790598255 cites W2330829410 @default.
• W2790598255 cites W2338227490 @default.
• W2790598255 cites W2344380565 @default.
• W2790598255 cites W2416717632 @default.
• W2790598255 cites W2517232211 @default.
• W2790598255 cites W2522491625 @default.
• W2790598255 cites W2526151113 @default.
• W2790598255 cites W2598334569 @default.
• W2790598255 cites W2600092497 @default.
• W2790598255 cites W2608422025 @default.
• W2790598255 cites W2685993713 @default.
• W2790598255 cites W2727201926 @default.
• W2790598255 cites W2747757902 @default.
• W2790598255 cites W59371674 @default.
• W2790598255 doi "https://doi.org/10.1128/jb.00006-18" @default.
• W2790598255 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5869481" @default.
• W2790598255 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29378892" @default.
• W2790598255 hasPublicationYear "2018" @default.
• W2790598255 type Work @default.
• W2790598255 sameAs 2790598255 @default.
• W2790598255 citedByCount "14" @default.
• W2790598255 countsByYear W27905982552018 @default.
• W2790598255 countsByYear W27905982552020 @default.
• W2790598255 countsByYear W27905982552021 @default.

• next