FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2790730158> ?p ?o ?g. }

• W2790730158 abstract "Recently, there have been several attempts to produce long-chain dicarboxylic acids (DCAs) in various microbial hosts. Of these, Yarrowia lipolytica has great potential due to its oleaginous characteristics and unique ability to utilize hydrophobic substrates. However, Y. lipolytica should be further engineered to make it more competitive: the current approaches are mostly intuitive and cumbersome, thus limiting its industrial application. In this study, we proposed model-guided metabolic engineering strategies for enhanced production of DCAs in Y. lipolytica. At the outset, we reconstructed genome-scale metabolic model (GSMM) of Y. lipolytica (iYLI647) by substantially expanding the previous models. Subsequently, the model was validated using three sets of published culture experiment data. It was finally exploited to identify genetic engineering targets for overexpression, knockout, and cofactor modification by applying several in silico strain design methods, which potentially give rise to high yield production of the industrially relevant long-chain DCAs, e.g., dodecanedioic acid (DDDA). The resultant targets include (1) malate dehydrogenase and malic enzyme genes and (2) glutamate dehydrogenase gene, in silico overexpression of which generated additional NADPH required for fatty acid synthesis, leading to the increased DDDA fluxes by 48% and 22% higher, respectively, compared to wild-type. We further investigated the effect of supplying branched-chain amino acids on the acetyl-CoA turn-over rate which is key metabolite for fatty acid synthesis, suggesting their significance for production of DDDA in Y. lipolytica. In silico model-based strain design strategies allowed us to identify several metabolic engineering targets for overproducing DCAs in lipid accumulating yeast, Y. lipolytica. Thus, the current study can provide a methodological framework that is applicable to other oleaginous yeasts for value-added biochemical production." @default.
• W2790730158 created "2018-03-29" @default.
• W2790730158 creator A5011206845 @default.
• W2790730158 creator A5029580669 @default.
• W2790730158 creator A5052315121 @default.
• W2790730158 creator A5058007321 @default.
• W2790730158 creator A5081133318 @default.
• W2790730158 creator A5091567098 @default.
• W2790730158 date "2018-03-01" @default.
• W2790730158 modified "2023-10-14" @default.
• W2790730158 title "Genome-scale model-driven strain design for dicarboxylic acid production in Yarrowia lipolytica" @default.
• W2790730158 cites W1220320927 @default.
• W2790730158 cites W1821513802 @default.
• W2790730158 cites W1967084983 @default.
• W2790730158 cites W1968589180 @default.
• W2790730158 cites W1970351449 @default.
• W2790730158 cites W1976247849 @default.
• W2790730158 cites W1984096521 @default.
• W2790730158 cites W1988694126 @default.
• W2790730158 cites W1992844569 @default.
• W2790730158 cites W1996355791 @default.
• W2790730158 cites W2003029675 @default.
• W2790730158 cites W2003686438 @default.
• W2790730158 cites W2007996759 @default.
• W2790730158 cites W2009163705 @default.
• W2790730158 cites W2012746361 @default.
• W2790730158 cites W2018915837 @default.
• W2790730158 cites W2040716173 @default.
• W2790730158 cites W2043028577 @default.
• W2790730158 cites W2048214825 @default.
• W2790730158 cites W2056305771 @default.
• W2790730158 cites W2057945124 @default.
• W2790730158 cites W2059004353 @default.
• W2790730158 cites W2062819025 @default.
• W2790730158 cites W2067317565 @default.
• W2790730158 cites W2073837631 @default.
• W2790730158 cites W2076830784 @default.
• W2790730158 cites W2078355959 @default.
• W2790730158 cites W2081623272 @default.
• W2790730158 cites W2085909480 @default.
• W2790730158 cites W2086592553 @default.
• W2790730158 cites W2093063735 @default.
• W2790730158 cites W2093941784 @default.
• W2790730158 cites W2096202610 @default.
• W2790730158 cites W2098824895 @default.
• W2790730158 cites W2101127697 @default.
• W2790730158 cites W2106576546 @default.
• W2790730158 cites W2108210068 @default.
• W2790730158 cites W2114417330 @default.
• W2790730158 cites W2116875566 @default.
• W2790730158 cites W2120924807 @default.
• W2790730158 cites W2128089862 @default.
• W2790730158 cites W2135081566 @default.
• W2790730158 cites W2140874470 @default.
• W2790730158 cites W2147472054 @default.
• W2790730158 cites W2152428375 @default.
• W2790730158 cites W2154797402 @default.
• W2790730158 cites W2158721838 @default.
• W2790730158 cites W2159408188 @default.
• W2790730158 cites W2164733077 @default.
• W2790730158 cites W2170487377 @default.
• W2790730158 cites W2170568766 @default.
• W2790730158 cites W2178907415 @default.
• W2790730158 cites W2205785247 @default.
• W2790730158 cites W2213256540 @default.
• W2790730158 cites W2312868359 @default.
• W2790730158 cites W2324359686 @default.
• W2790730158 cites W2333535089 @default.
• W2790730158 doi "https://doi.org/10.1186/s12918-018-0542-5" @default.
• W2790730158 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5861505" @default.
• W2790730158 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29560822" @default.
• W2790730158 hasPublicationYear "2018" @default.
• W2790730158 type Work @default.
• W2790730158 sameAs 2790730158 @default.
• W2790730158 citedByCount "51" @default.
• W2790730158 countsByYear W27907301582018 @default.
• W2790730158 countsByYear W27907301582019 @default.
• W2790730158 countsByYear W27907301582020 @default.
• W2790730158 countsByYear W27907301582021 @default.
• W2790730158 countsByYear W27907301582022 @default.
• W2790730158 countsByYear W27907301582023 @default.
• W2790730158 crossrefType "journal-article" @default.
• W2790730158 hasAuthorship W2790730158A5011206845 @default.
• W2790730158 hasAuthorship W2790730158A5029580669 @default.
• W2790730158 hasAuthorship W2790730158A5052315121 @default.
• W2790730158 hasAuthorship W2790730158A5058007321 @default.
• W2790730158 hasAuthorship W2790730158A5081133318 @default.
• W2790730158 hasAuthorship W2790730158A5091567098 @default.
• W2790730158 hasBestOaLocation W27907301581 @default.
• W2790730158 hasConcept C104317684 @default.
• W2790730158 hasConcept C191908910 @default.
• W2790730158 hasConcept C192989942 @default.
• W2790730158 hasConcept C2775905019 @default.
• W2790730158 hasConcept C2779222958 @default.
• W2790730158 hasConcept C2779490540 @default.
• W2790730158 hasConcept C543025807 @default.
• W2790730158 hasConcept C55493867 @default.
• W2790730158 hasConcept C6350086 @default.
• W2790730158 hasConcept C70721500 @default.
• W2790730158 hasConcept C77957584 @default.

• next