Matches in SemOpenAlex for { <https://semopenalex.org/work/W2790865582> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2790865582 endingPage "864" @default.
- W2790865582 startingPage "858" @default.
- W2790865582 abstract "A key challenge in the development of novel chemotherapeutics is the design of molecules capable of selective toxicity to cancer cells. Antibodies have greater target specificity compared to small molecule drugs, but most are unable to penetrate cells, and predominantly target extracellular antigens. A nuclear-penetrating anti-DNA autoantibody isolated from the MRL/lpr lupus mouse model, 3E10, preferentially localizes to tumors, inhibits DNA repair, and selectively kills cancer cells with defects in DNA repair. A murine divalent single chain variable fragment of 3E10 with mutations for improved DNA binding affinity, 3E10 (D31N) di-scFv, has previously been produced in P. pastoris and yielded promising pre-clinical findings, but is unsuitable for clinical testing. The present study reports the design, expression and testing of a panel of humanized 3E10 (D31N) di-scFvs, some of which contain CDR substitution. These variants were expressed in a modified CHO system and evaluated for their physicochemical attributes and ability to penetrate nuclei to selectively cause DNA damage accumulation in and kill cancer cells with DNA repair defects. Secondary structure was conserved and most variants retained the key characteristics of the murine 3E10 (D31N) di-scFv produced in P. pastoris. Moreover, several variants with CDR substitutions outperformed the murine prototype. In conclusion, we have designed several humanized variants of 3E10 (D31N) di-scFv that have potential for application as monotherapy or conjugates for targeted nuclear drug delivery." @default.
- W2790865582 created "2018-03-29" @default.
- W2790865582 creator A5006765542 @default.
- W2790865582 creator A5024000145 @default.
- W2790865582 creator A5033145648 @default.
- W2790865582 creator A5039272261 @default.
- W2790865582 creator A5059167331 @default.
- W2790865582 creator A5069096450 @default.
- W2790865582 creator A5088345503 @default.
- W2790865582 date "2018-02-01" @default.
- W2790865582 modified "2023-09-26" @default.
- W2790865582 title "Re-engineering and evaluation of anti-DNA autoantibody 3E10 for therapeutic applications" @default.
- W2790865582 cites W1588979652 @default.
- W2790865582 cites W1803102843 @default.
- W2790865582 cites W1979573410 @default.
- W2790865582 cites W1995946547 @default.
- W2790865582 cites W2043228552 @default.
- W2790865582 cites W2045658449 @default.
- W2790865582 cites W2051912329 @default.
- W2790865582 cites W2104623056 @default.
- W2790865582 cites W2113985551 @default.
- W2790865582 cites W2120744225 @default.
- W2790865582 cites W2121213878 @default.
- W2790865582 cites W2143724468 @default.
- W2790865582 cites W2156343873 @default.
- W2790865582 cites W2161199282 @default.
- W2790865582 cites W2179836657 @default.
- W2790865582 cites W2307755391 @default.
- W2790865582 cites W4240597302 @default.
- W2790865582 cites W793363133 @default.
- W2790865582 doi "https://doi.org/10.1016/j.bbrc.2018.01.139" @default.
- W2790865582 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29374508" @default.
- W2790865582 hasPublicationYear "2018" @default.
- W2790865582 type Work @default.
- W2790865582 sameAs 2790865582 @default.
- W2790865582 citedByCount "11" @default.
- W2790865582 countsByYear W27908655822018 @default.
- W2790865582 countsByYear W27908655822019 @default.
- W2790865582 countsByYear W27908655822020 @default.
- W2790865582 countsByYear W27908655822021 @default.
- W2790865582 countsByYear W27908655822022 @default.
- W2790865582 countsByYear W27908655822023 @default.
- W2790865582 crossrefType "journal-article" @default.
- W2790865582 hasAuthorship W2790865582A5006765542 @default.
- W2790865582 hasAuthorship W2790865582A5024000145 @default.
- W2790865582 hasAuthorship W2790865582A5033145648 @default.
- W2790865582 hasAuthorship W2790865582A5039272261 @default.
- W2790865582 hasAuthorship W2790865582A5059167331 @default.
- W2790865582 hasAuthorship W2790865582A5069096450 @default.
- W2790865582 hasAuthorship W2790865582A5088345503 @default.
- W2790865582 hasBestOaLocation W27908655822 @default.
- W2790865582 hasConcept C104317684 @default.
- W2790865582 hasConcept C143425029 @default.
- W2790865582 hasConcept C153911025 @default.
- W2790865582 hasConcept C185592680 @default.
- W2790865582 hasConcept C2776967038 @default.
- W2790865582 hasConcept C40767141 @default.
- W2790865582 hasConcept C502942594 @default.
- W2790865582 hasConcept C552990157 @default.
- W2790865582 hasConcept C55493867 @default.
- W2790865582 hasConcept C86803240 @default.
- W2790865582 hasConcept C95444343 @default.
- W2790865582 hasConceptScore W2790865582C104317684 @default.
- W2790865582 hasConceptScore W2790865582C143425029 @default.
- W2790865582 hasConceptScore W2790865582C153911025 @default.
- W2790865582 hasConceptScore W2790865582C185592680 @default.
- W2790865582 hasConceptScore W2790865582C2776967038 @default.
- W2790865582 hasConceptScore W2790865582C40767141 @default.
- W2790865582 hasConceptScore W2790865582C502942594 @default.
- W2790865582 hasConceptScore W2790865582C552990157 @default.
- W2790865582 hasConceptScore W2790865582C55493867 @default.
- W2790865582 hasConceptScore W2790865582C86803240 @default.
- W2790865582 hasConceptScore W2790865582C95444343 @default.
- W2790865582 hasFunder F4320309118 @default.
- W2790865582 hasIssue "3" @default.
- W2790865582 hasLocation W27908655821 @default.
- W2790865582 hasLocation W27908655822 @default.
- W2790865582 hasLocation W27908655823 @default.
- W2790865582 hasOpenAccess W2790865582 @default.
- W2790865582 hasPrimaryLocation W27908655821 @default.
- W2790865582 hasRelatedWork W1499193777 @default.
- W2790865582 hasRelatedWork W1983626749 @default.
- W2790865582 hasRelatedWork W2003457467 @default.
- W2790865582 hasRelatedWork W2028633235 @default.
- W2790865582 hasRelatedWork W2112881545 @default.
- W2790865582 hasRelatedWork W2116860088 @default.
- W2790865582 hasRelatedWork W2424758321 @default.
- W2790865582 hasRelatedWork W2769377618 @default.
- W2790865582 hasRelatedWork W4230296311 @default.
- W2790865582 hasRelatedWork W4283706870 @default.
- W2790865582 hasVolume "496" @default.
- W2790865582 isParatext "false" @default.
- W2790865582 isRetracted "false" @default.
- W2790865582 magId "2790865582" @default.
- W2790865582 workType "article" @default.