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- W2791041630 abstract "Droplet Interface Bilayers (DIBs) created at water/organic solvent interfaces are becoming more and more popular as tools for investigating the biophysical properties of membranes and transmembrane transporters. The current approaches for DIB preparation include the formation of a small spherical droplet around a hydrogel support. While the stability of the formed membrane is substantially increased by the support addition, the same support impedes any further changes in electrolyte composition, droplet size, later addition of molecules targeting the membrane, or a complete buffer exchange. In this work, we eliminated these shortcomings by creating DIBs without using a solid support. The formation of the water droplet at the fine tip of a glass pipette enabled production of very stable bilayer membranes at the water/oil interface. The solution composition was easily adjusted with a microfluidic injection system, which also allowed further addition of the pore-forming protein lysenin for full reconstitution in the membrane. The functionality of inserted lysenin channels was assessed from their response to regulatory stimuli such as voltage and multivalent metal ions used as ligands. The system was completed with a nanofluidic magnetic pump which allowed precise control of the droplet size and membrane surface area, as inferred from microscopy imaging and electrophysiology experiments. In conclusion, unsupported DIBs may constitute a powerful tool for investigating the biological activity of transporters, along with fundamental physical features of bilayer lipid membranes." @default.
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- W2791041630 date "2018-02-01" @default.
- W2791041630 modified "2023-10-18" @default.
- W2791041630 title "Lysenin Channel Reconstitution into Unsupported Droplet Interface Bilayers" @default.
- W2791041630 doi "https://doi.org/10.1016/j.bpj.2017.11.1530" @default.
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