Matches in SemOpenAlex for { <https://semopenalex.org/work/W2791513037> ?p ?o ?g. }
- W2791513037 abstract "Phage display is a technique by which peptides are displayed on the surface of phage. The development of a novel phage display strategy, cosmix-plexing, allows the isolation of high affinity ligands. Cosmix-plexing allows recombination within hypervariable DNA regions, due to type IIs enzyme which bind at a defined sequence, but cleave a fixed distance away. This technique allows the reassortment of the right and left sections of hypervariable domains, and therefore increases the initial diversity of the library, or optimizes the binding capacities of a preselected population. A dedicated biased cosmix-plexing library for SH3 domains was designed, which contained three Pro at fixed positions, since SH3 domains show high preference for Pro-rich ligands. EVH1 domains also bind Pro-rich ligands and were also considered as a target for the library. High affinity ligands were isolated. BIAcore analyses were performed, which demonstrated that the KD of these ligands are in the nM range, i.e. their affinity for SH3 domains are 1000 fold higher than previously described SH3 binders. Such high affinity ligands allow the development of tools to investigate the function of SH3 domain in the signal transduction cascade, where SH3 natural ligands might be competed away in vivo, to tightly control and regulate the signal transduction. Preliminary experiments to deliver our SH3 peptides into living cells, using the Tat-fusion protein transduction system, allow optimism regarding these models." @default.
- W2791513037 created "2018-03-29" @default.
- W2791513037 creator A5005374502 @default.
- W2791513037 date "2000-06-06" @default.
- W2791513037 modified "2023-09-24" @default.
- W2791513037 title "Isolation of high affinity ligands against SH3 and EVH1 domains using the phage display cosmix-plexing technology" @default.
- W2791513037 cites W1004959057 @default.
- W2791513037 cites W116322834 @default.
- W2791513037 cites W11949986 @default.
- W2791513037 cites W126048801 @default.
- W2791513037 cites W1434255190 @default.
- W2791513037 cites W1485730287 @default.
- W2791513037 cites W1486876329 @default.
- W2791513037 cites W1487704589 @default.
- W2791513037 cites W1489538658 @default.
- W2791513037 cites W1491028479 @default.
- W2791513037 cites W1503880077 @default.
- W2791513037 cites W1515543767 @default.
- W2791513037 cites W1522400918 @default.
- W2791513037 cites W1535318109 @default.
- W2791513037 cites W1542067597 @default.
- W2791513037 cites W1543240545 @default.
- W2791513037 cites W1544995612 @default.
- W2791513037 cites W1545653070 @default.
- W2791513037 cites W1546632541 @default.
- W2791513037 cites W1547924576 @default.
- W2791513037 cites W1549274675 @default.
- W2791513037 cites W1555021163 @default.
- W2791513037 cites W1555742321 @default.
- W2791513037 cites W1557290263 @default.
- W2791513037 cites W1560280455 @default.
- W2791513037 cites W1560560243 @default.
- W2791513037 cites W1564113586 @default.
- W2791513037 cites W1576016596 @default.
- W2791513037 cites W1585344957 @default.
- W2791513037 cites W1597888074 @default.
- W2791513037 cites W1606431180 @default.
- W2791513037 cites W1607258414 @default.
- W2791513037 cites W1662447390 @default.
- W2791513037 cites W1676103704 @default.
- W2791513037 cites W1719330231 @default.
- W2791513037 cites W1796176673 @default.
- W2791513037 cites W1806791563 @default.
- W2791513037 cites W1835713486 @default.
- W2791513037 cites W1873199501 @default.
- W2791513037 cites W1923801572 @default.
- W2791513037 cites W1965261376 @default.
- W2791513037 cites W1965392409 @default.
- W2791513037 cites W1965583590 @default.
- W2791513037 cites W1965946471 @default.
- W2791513037 cites W1966167062 @default.
- W2791513037 cites W1966761076 @default.
- W2791513037 cites W1967345709 @default.
- W2791513037 cites W1967448808 @default.
- W2791513037 cites W1968250691 @default.
- W2791513037 cites W1969595441 @default.
- W2791513037 cites W1969943007 @default.
- W2791513037 cites W1970046422 @default.
- W2791513037 cites W1971342904 @default.
- W2791513037 cites W1971377120 @default.
- W2791513037 cites W1971447541 @default.
- W2791513037 cites W1973019272 @default.
- W2791513037 cites W1973569817 @default.
- W2791513037 cites W1974145192 @default.
- W2791513037 cites W1974956422 @default.
- W2791513037 cites W1977186059 @default.
- W2791513037 cites W1978331283 @default.
- W2791513037 cites W1978687607 @default.
- W2791513037 cites W1980601547 @default.
- W2791513037 cites W1980602424 @default.
- W2791513037 cites W1980738032 @default.
- W2791513037 cites W1981371363 @default.
- W2791513037 cites W1981837069 @default.
- W2791513037 cites W1982149924 @default.
- W2791513037 cites W1982186905 @default.
- W2791513037 cites W1982849870 @default.
- W2791513037 cites W1984919127 @default.
- W2791513037 cites W1985057592 @default.
- W2791513037 cites W1987349623 @default.
- W2791513037 cites W1987414591 @default.
- W2791513037 cites W1987823655 @default.
- W2791513037 cites W1987888718 @default.
- W2791513037 cites W1987889377 @default.
- W2791513037 cites W1988351115 @default.
- W2791513037 cites W1988760345 @default.
- W2791513037 cites W1989842486 @default.
- W2791513037 cites W1989987282 @default.
- W2791513037 cites W1990516841 @default.
- W2791513037 cites W1991683855 @default.
- W2791513037 cites W1992569957 @default.
- W2791513037 cites W1993520656 @default.
- W2791513037 cites W1993742172 @default.
- W2791513037 cites W1994240328 @default.
- W2791513037 cites W1994652908 @default.
- W2791513037 cites W1995498328 @default.
- W2791513037 cites W1995976925 @default.
- W2791513037 cites W1997718936 @default.
- W2791513037 cites W1997859241 @default.
- W2791513037 cites W1998544105 @default.
- W2791513037 cites W2000170431 @default.