FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2791521637> ?p ?o ?g. }

• W2791521637 endingPage "5908" @default.
• W2791521637 startingPage "5895" @default.
• W2791521637 abstract "The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that is an integral component of proliferative signaling. EGFRs on the cell surface become activated upon EGF binding and have an increased rate of endocytosis. Once in the cytoplasm, the EGF·EGFR complex is trafficked to the lysosome for degradation, and signaling is terminated. During trafficking, the EGFR kinase domain remains active, and the internalized EGFR can continue signaling to downstream effectors. Although effector activity varies based on the EGFR's endocytic location, it is not clear how this occurs. In an effort to identify proteins that uniquely associate with the internalized, liganded EGFR in the early endosome, we developed an early endosome isolation strategy to analyze their protein composition. Post-nuclear supernatant from HeLa cells stimulated with and without EGF were separated on an isotonic 17% Percoll gradient. The gradient was fractionated, and early endosomal fractions were pooled and immunoisolated with an EEA1 mAb. The isolated endosomes were validated by immunoblot using antibodies against organelle-specific marker proteins and transmission EM. These early endosomes were also subjected to LC-MS/MS for proteomic analysis. Five proteins were detected in endosomes in a ligand-dependent manner: EGFR, RUFY1, STOML2, PTPN23, and CCDC51. Knockdown of RUFY1 or PTPN23 by RNAi indicated that both proteins play a role in EGFR trafficking. These experiments indicate that endocytic trafficking of activated EGFR changes the protein composition, membrane trafficking, and signaling potential of the early endosome." @default.
• W2791521637 created "2018-03-29" @default.
• W2791521637 creator A5017157522 @default.
• W2791521637 creator A5053273455 @default.
• W2791521637 creator A5073124045 @default.
• W2791521637 creator A5085061817 @default.
• W2791521637 date "2018-04-01" @default.
• W2791521637 modified "2023-10-12" @default.
• W2791521637 title "Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor–dependent manner" @default.
• W2791521637 cites W1492722920 @default.
• W2791521637 cites W1505339870 @default.
• W2791521637 cites W1587854090 @default.
• W2791521637 cites W1669303234 @default.
• W2791521637 cites W1965458495 @default.
• W2791521637 cites W1966658428 @default.
• W2791521637 cites W1975389753 @default.
• W2791521637 cites W1975788909 @default.
• W2791521637 cites W1982508870 @default.
• W2791521637 cites W1984186782 @default.
• W2791521637 cites W1984536659 @default.
• W2791521637 cites W1991366673 @default.
• W2791521637 cites W1995499011 @default.
• W2791521637 cites W1999328455 @default.
• W2791521637 cites W1999981814 @default.
• W2791521637 cites W2001609281 @default.
• W2791521637 cites W2002096723 @default.
• W2791521637 cites W2011642803 @default.
• W2791521637 cites W2015172381 @default.
• W2791521637 cites W2016923195 @default.
• W2791521637 cites W2025356688 @default.
• W2791521637 cites W2043162174 @default.
• W2791521637 cites W2049713657 @default.
• W2791521637 cites W2051120695 @default.
• W2791521637 cites W2051919508 @default.
• W2791521637 cites W2052705131 @default.
• W2791521637 cites W2054735667 @default.
• W2791521637 cites W2059418306 @default.
• W2791521637 cites W2061671663 @default.
• W2791521637 cites W2071967953 @default.
• W2791521637 cites W2072371514 @default.
• W2791521637 cites W2088284664 @default.
• W2791521637 cites W2094540897 @default.
• W2791521637 cites W2106223472 @default.
• W2791521637 cites W2110303521 @default.
• W2791521637 cites W2111622584 @default.
• W2791521637 cites W2113771101 @default.
• W2791521637 cites W2121158677 @default.
• W2791521637 cites W2122223062 @default.
• W2791521637 cites W2132782300 @default.
• W2791521637 cites W2133051701 @default.
• W2791521637 cites W2137513862 @default.
• W2791521637 cites W2144319052 @default.
• W2791521637 cites W2145950214 @default.
• W2791521637 cites W2147493949 @default.
• W2791521637 cites W2150419742 @default.
• W2791521637 cites W2187659172 @default.
• W2791521637 cites W2467443457 @default.
• W2791521637 cites W2742300367 @default.
• W2791521637 cites W4903968 @default.
• W2791521637 doi "https://doi.org/10.1074/jbc.ra117.000632" @default.
• W2791521637 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5912451" @default.
• W2791521637 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29523688" @default.
• W2791521637 hasPublicationYear "2018" @default.
• W2791521637 type Work @default.
• W2791521637 sameAs 2791521637 @default.
• W2791521637 citedByCount "20" @default.
• W2791521637 countsByYear W27915216372018 @default.
• W2791521637 countsByYear W27915216372019 @default.
• W2791521637 countsByYear W27915216372020 @default.
• W2791521637 countsByYear W27915216372021 @default.
• W2791521637 countsByYear W27915216372022 @default.
• W2791521637 countsByYear W27915216372023 @default.
• W2791521637 crossrefType "journal-article" @default.
• W2791521637 hasAuthorship W2791521637A5017157522 @default.
• W2791521637 hasAuthorship W2791521637A5053273455 @default.
• W2791521637 hasAuthorship W2791521637A5073124045 @default.
• W2791521637 hasAuthorship W2791521637A5085061817 @default.
• W2791521637 hasBestOaLocation W27915216371 @default.
• W2791521637 hasConcept C102747710 @default.
• W2791521637 hasConcept C104317684 @default.
• W2791521637 hasConcept C170493617 @default.
• W2791521637 hasConcept C185592680 @default.
• W2791521637 hasConcept C2776362946 @default.
• W2791521637 hasConcept C46111723 @default.
• W2791521637 hasConcept C55493867 @default.
• W2791521637 hasConcept C70721500 @default.
• W2791521637 hasConcept C79879829 @default.
• W2791521637 hasConcept C86803240 @default.
• W2791521637 hasConcept C95444343 @default.
• W2791521637 hasConceptScore W2791521637C102747710 @default.
• W2791521637 hasConceptScore W2791521637C104317684 @default.
• W2791521637 hasConceptScore W2791521637C170493617 @default.
• W2791521637 hasConceptScore W2791521637C185592680 @default.
• W2791521637 hasConceptScore W2791521637C2776362946 @default.
• W2791521637 hasConceptScore W2791521637C46111723 @default.
• W2791521637 hasConceptScore W2791521637C55493867 @default.
• W2791521637 hasConceptScore W2791521637C70721500 @default.
• W2791521637 hasConceptScore W2791521637C79879829 @default.

• next