Matches in SemOpenAlex for { <https://semopenalex.org/work/W2791758284> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2791758284 endingPage "385a" @default.
- W2791758284 startingPage "385a" @default.
- W2791758284 abstract "Protein folding under force does mechanical work, however, for that work to be physiologically relevant, it must be delivered fast enough to compete with the speed of molecular motors. For instance, it is not known if titin domains can fold fast enough to follow the myosin motors, thus contributing to the mechanical power during muscle contraction. Accurate measurement of the initial folding velocity requires applying fast force changes in order to resolve the initial folding events. Yet, most force-spectroscopy instruments change the force by moving mechanical components, introducing a time lag-at least 100 ms in the case of magnetic tweezers-where these early molecular events are lost. Here, we present a newly developed electromagnetic tweezers instrument, where the magnetic field is generated by an electromagnetic head. The force acting on the superparamagnetic beads depends on the electric current flowing through the head, and can therefore be changed as fast as current can be supplied. Based on Karlqvist approximation of the field created by an electromagnetic head, we work out a closed analytical expression that relates the force on the superparamagnetic beads with the electric current and the vertical distance of the head to the bead. We calibrate our instrument by relating this force expression with the step-sizes of protein L unfolding/refolding events, allowing to reach forces of 50 pN in 5 ms, when working at a distance of 200 μm and an electric current of 600 mA. This force range can potentially be increased up to 500 pN by using thinner fluid chambers, approaching the AFM force range, but with the exquisite stability of magnetic tweezers. Our instrument development offers a unique tool for studying how proteins respond mechanically to force perturbations as those experienced in vivo." @default.
- W2791758284 created "2018-03-29" @default.
- W2791758284 creator A5030514973 @default.
- W2791758284 creator A5034142451 @default.
- W2791758284 creator A5049272540 @default.
- W2791758284 date "2018-02-01" @default.
- W2791758284 modified "2023-09-28" @default.
- W2791758284 title "An Electromagnetic Tweezers for Studying Fast Protein Folding Dynamics" @default.
- W2791758284 doi "https://doi.org/10.1016/j.bpj.2017.11.2129" @default.
- W2791758284 hasPublicationYear "2018" @default.
- W2791758284 type Work @default.
- W2791758284 sameAs 2791758284 @default.
- W2791758284 citedByCount "1" @default.
- W2791758284 countsByYear W27917582842021 @default.
- W2791758284 crossrefType "journal-article" @default.
- W2791758284 hasAuthorship W2791758284A5030514973 @default.
- W2791758284 hasAuthorship W2791758284A5034142451 @default.
- W2791758284 hasAuthorship W2791758284A5049272540 @default.
- W2791758284 hasBestOaLocation W27917582841 @default.
- W2791758284 hasConcept C102951782 @default.
- W2791758284 hasConcept C105480513 @default.
- W2791758284 hasConcept C115260700 @default.
- W2791758284 hasConcept C119473381 @default.
- W2791758284 hasConcept C120665830 @default.
- W2791758284 hasConcept C121332964 @default.
- W2791758284 hasConcept C127413603 @default.
- W2791758284 hasConcept C134018914 @default.
- W2791758284 hasConcept C171250308 @default.
- W2791758284 hasConcept C185592680 @default.
- W2791758284 hasConcept C18762648 @default.
- W2791758284 hasConcept C192562407 @default.
- W2791758284 hasConcept C20198109 @default.
- W2791758284 hasConcept C207200792 @default.
- W2791758284 hasConcept C2776545253 @default.
- W2791758284 hasConcept C28843909 @default.
- W2791758284 hasConcept C2993110782 @default.
- W2791758284 hasConcept C43617362 @default.
- W2791758284 hasConcept C57879066 @default.
- W2791758284 hasConcept C60799052 @default.
- W2791758284 hasConcept C62520636 @default.
- W2791758284 hasConcept C68731436 @default.
- W2791758284 hasConcept C71924100 @default.
- W2791758284 hasConcept C78519656 @default.
- W2791758284 hasConcept C78902741 @default.
- W2791758284 hasConcept C90671521 @default.
- W2791758284 hasConcept C97355855 @default.
- W2791758284 hasConceptScore W2791758284C102951782 @default.
- W2791758284 hasConceptScore W2791758284C105480513 @default.
- W2791758284 hasConceptScore W2791758284C115260700 @default.
- W2791758284 hasConceptScore W2791758284C119473381 @default.
- W2791758284 hasConceptScore W2791758284C120665830 @default.
- W2791758284 hasConceptScore W2791758284C121332964 @default.
- W2791758284 hasConceptScore W2791758284C127413603 @default.
- W2791758284 hasConceptScore W2791758284C134018914 @default.
- W2791758284 hasConceptScore W2791758284C171250308 @default.
- W2791758284 hasConceptScore W2791758284C185592680 @default.
- W2791758284 hasConceptScore W2791758284C18762648 @default.
- W2791758284 hasConceptScore W2791758284C192562407 @default.
- W2791758284 hasConceptScore W2791758284C20198109 @default.
- W2791758284 hasConceptScore W2791758284C207200792 @default.
- W2791758284 hasConceptScore W2791758284C2776545253 @default.
- W2791758284 hasConceptScore W2791758284C28843909 @default.
- W2791758284 hasConceptScore W2791758284C2993110782 @default.
- W2791758284 hasConceptScore W2791758284C43617362 @default.
- W2791758284 hasConceptScore W2791758284C57879066 @default.
- W2791758284 hasConceptScore W2791758284C60799052 @default.
- W2791758284 hasConceptScore W2791758284C62520636 @default.
- W2791758284 hasConceptScore W2791758284C68731436 @default.
- W2791758284 hasConceptScore W2791758284C71924100 @default.
- W2791758284 hasConceptScore W2791758284C78519656 @default.
- W2791758284 hasConceptScore W2791758284C78902741 @default.
- W2791758284 hasConceptScore W2791758284C90671521 @default.
- W2791758284 hasConceptScore W2791758284C97355855 @default.
- W2791758284 hasIssue "3" @default.
- W2791758284 hasLocation W27917582841 @default.
- W2791758284 hasOpenAccess W2791758284 @default.
- W2791758284 hasPrimaryLocation W27917582841 @default.
- W2791758284 hasRelatedWork W1764424065 @default.
- W2791758284 hasRelatedWork W2002065705 @default.
- W2791758284 hasRelatedWork W2040617365 @default.
- W2791758284 hasRelatedWork W2474572737 @default.
- W2791758284 hasRelatedWork W2593123705 @default.
- W2791758284 hasRelatedWork W2798304139 @default.
- W2791758284 hasRelatedWork W3119748162 @default.
- W2791758284 hasRelatedWork W3209202935 @default.
- W2791758284 hasRelatedWork W3217336066 @default.
- W2791758284 hasRelatedWork W4229506256 @default.
- W2791758284 hasVolume "114" @default.
- W2791758284 isParatext "false" @default.
- W2791758284 isRetracted "false" @default.
- W2791758284 magId "2791758284" @default.
- W2791758284 workType "article" @default.