Matches in SemOpenAlex for { <https://semopenalex.org/work/W2792692625> ?p ?o ?g. }
- W2792692625 endingPage "21" @default.
- W2792692625 startingPage "14" @default.
- W2792692625 abstract "To improve the thermostability of α-L-rhamnosidase (r-Rha1), an enzyme previously identified from Aspergillus niger JMU-TS528, multiple arginine (Arg) residues were introduced into the r-Rha1 sequence to replace several lysine (Lys) residues that located on the surface of the folded r-Rha1. Hinted by in silico analysis, five surface Lys residues (K134, K228, K406, K440, K573) were targeted to produce a list of 5 single-residue mutants and 4 multiple-residue mutants using site-directed mutagenesis. Among these mutants, a double Lys to Arg mutant, i.e. K406R/K573R, showed the best thermostability improvement. The half-life of this mutant's enzyme activity increased 3 h at 60 °C, 23 min at 65 °C, and 3.5 min at 70 °C, when compared with the wild type. The simulated protein structure based interaction analysis and molecular dynamics calculation indicate that the thermostability improvement of the mutant K406R-K573R was possibly due to the extra hydrogen bonds, the additional cation-π interactions, and the relatively compact conformation. With the enhanced thermostability, the α-L-rhamnosidase mutant, K406R-K573R, has potentially broadened the r-Rha1 applications in food processing industry." @default.
- W2792692625 created "2018-03-29" @default.
- W2792692625 creator A5001501699 @default.
- W2792692625 creator A5018744849 @default.
- W2792692625 creator A5029525238 @default.
- W2792692625 creator A5036413944 @default.
- W2792692625 creator A5036467914 @default.
- W2792692625 creator A5060425072 @default.
- W2792692625 creator A5063693964 @default.
- W2792692625 creator A5080998212 @default.
- W2792692625 creator A5089126336 @default.
- W2792692625 date "2018-06-01" @default.
- W2792692625 modified "2023-09-30" @default.
- W2792692625 title "Improving the thermostability by introduction of arginines on the surface of α-L-rhamnosidase (r-Rha1) from Aspergillus niger" @default.
- W2792692625 cites W1198386344 @default.
- W2792692625 cites W1850775773 @default.
- W2792692625 cites W1922854646 @default.
- W2792692625 cites W1965381459 @default.
- W2792692625 cites W1979520014 @default.
- W2792692625 cites W1979915001 @default.
- W2792692625 cites W1987241822 @default.
- W2792692625 cites W1994074483 @default.
- W2792692625 cites W1997143265 @default.
- W2792692625 cites W1998109996 @default.
- W2792692625 cites W2002267563 @default.
- W2792692625 cites W2014555485 @default.
- W2792692625 cites W2024502813 @default.
- W2792692625 cites W2034555311 @default.
- W2792692625 cites W2034935766 @default.
- W2792692625 cites W2036644638 @default.
- W2792692625 cites W2045608551 @default.
- W2792692625 cites W2051419400 @default.
- W2792692625 cites W2070213522 @default.
- W2792692625 cites W2077937415 @default.
- W2792692625 cites W2084823034 @default.
- W2792692625 cites W2090848871 @default.
- W2792692625 cites W2102934258 @default.
- W2792692625 cites W2107218115 @default.
- W2792692625 cites W2117297062 @default.
- W2792692625 cites W2123911471 @default.
- W2792692625 cites W2138270807 @default.
- W2792692625 cites W2140874656 @default.
- W2792692625 cites W2141954292 @default.
- W2792692625 cites W2144852755 @default.
- W2792692625 cites W2154665416 @default.
- W2792692625 cites W2158543301 @default.
- W2792692625 cites W2225560095 @default.
- W2792692625 cites W2271138693 @default.
- W2792692625 cites W2295369589 @default.
- W2792692625 cites W2300649977 @default.
- W2792692625 cites W2326025763 @default.
- W2792692625 cites W2328563325 @default.
- W2792692625 cites W2328961664 @default.
- W2792692625 cites W2335612086 @default.
- W2792692625 cites W2466253277 @default.
- W2792692625 cites W2589245531 @default.
- W2792692625 cites W2769787337 @default.
- W2792692625 cites W852222129 @default.
- W2792692625 doi "https://doi.org/10.1016/j.ijbiomac.2018.01.078" @default.
- W2792692625 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29355637" @default.
- W2792692625 hasPublicationYear "2018" @default.
- W2792692625 type Work @default.
- W2792692625 sameAs 2792692625 @default.
- W2792692625 citedByCount "17" @default.
- W2792692625 countsByYear W27926926252019 @default.
- W2792692625 countsByYear W27926926252020 @default.
- W2792692625 countsByYear W27926926252021 @default.
- W2792692625 countsByYear W27926926252022 @default.
- W2792692625 countsByYear W27926926252023 @default.
- W2792692625 crossrefType "journal-article" @default.
- W2792692625 hasAuthorship W2792692625A5001501699 @default.
- W2792692625 hasAuthorship W2792692625A5018744849 @default.
- W2792692625 hasAuthorship W2792692625A5029525238 @default.
- W2792692625 hasAuthorship W2792692625A5036413944 @default.
- W2792692625 hasAuthorship W2792692625A5036467914 @default.
- W2792692625 hasAuthorship W2792692625A5060425072 @default.
- W2792692625 hasAuthorship W2792692625A5063693964 @default.
- W2792692625 hasAuthorship W2792692625A5080998212 @default.
- W2792692625 hasAuthorship W2792692625A5089126336 @default.
- W2792692625 hasConcept C103408237 @default.
- W2792692625 hasConcept C104317684 @default.
- W2792692625 hasConcept C143065580 @default.
- W2792692625 hasConcept C181199279 @default.
- W2792692625 hasConcept C185592680 @default.
- W2792692625 hasConcept C2775905019 @default.
- W2792692625 hasConcept C2775960407 @default.
- W2792692625 hasConcept C2781216036 @default.
- W2792692625 hasConcept C2781338088 @default.
- W2792692625 hasConcept C55493867 @default.
- W2792692625 hasConcept C71240020 @default.
- W2792692625 hasConceptScore W2792692625C103408237 @default.
- W2792692625 hasConceptScore W2792692625C104317684 @default.
- W2792692625 hasConceptScore W2792692625C143065580 @default.
- W2792692625 hasConceptScore W2792692625C181199279 @default.
- W2792692625 hasConceptScore W2792692625C185592680 @default.
- W2792692625 hasConceptScore W2792692625C2775905019 @default.
- W2792692625 hasConceptScore W2792692625C2775960407 @default.
- W2792692625 hasConceptScore W2792692625C2781216036 @default.