FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2792727468> ?p ?o ?g. }

• W2792727468 abstract "ABSTRACT While designing synthetic adhesives that perform in aqueous environments has proven challenging, microorganisms commonly produce bioadhesives that efficiently attach to a variety of substrates, including wet surfaces. The aquatic bacterium Caulobacter crescentus uses a discrete polysaccharide complex, the holdfast, to strongly attach to surfaces and resist flow. The holdfast is extremely versatile and has impressive adhesive strength. Here, we used atomic force microscopy in conjunction with superresolution microscopy and enzymatic assays to unravel the complex structure of the holdfast and to characterize its chemical constituents and their role in adhesion. Our data support a model whereby the holdfast is a heterogeneous material organized as two layers: a stiffer nanoscopic core layer wrapped into a sparse, far-reaching, flexible brush layer. Moreover, we found that the elastic response of the holdfast evolves after surface contact from initially heterogeneous to more homogeneous. From a composition point of view, besides N -acetyl- d -glucosamine (NAG), the only component that had been identified to date, our data show that the holdfast contains peptides and DNA. We hypothesize that, while polypeptides are the most important components for adhesive force, the presence of DNA mainly impacts the brush layer and the strength of initial adhesion, with NAG playing a primarily structural role within the core. The unanticipated complexity of both the structure and composition of the holdfast likely underlies its versatility as a wet adhesive and its distinctive strength. Continued improvements in understanding of the mechanochemistry of this bioadhesive could provide new insights into how bacteria attach to surfaces and could inform the development of new adhesives. IMPORTANCE There is an urgent need for strong, biocompatible bioadhesives that perform underwater. To strongly adhere to surfaces and resist flow underwater, the bacterium Caulobacter crescentus produces an adhesive called the holdfast, the mechanochemistry of which remains undefined. We show that the holdfast is a layered structure with a stiff core layer and a polymeric brush layer and consists of polysaccharides, polypeptides, and DNA. The DNA appears to play a role in the structure of the brush layer and initial adhesion, the peptides in adhesive strength, and the polysaccharides in the structure of the core. The complex, multilayer organization and diverse chemistry described here underlie the distinctive adhesive properties of the holdfast and will provide important insights into the mechanisms of bacterial adhesion and bioadhesive applications." @default.
• W2792727468 created "2018-03-29" @default.
• W2792727468 creator A5019167221 @default.
• W2792727468 creator A5025176275 @default.
• W2792727468 creator A5032388146 @default.
• W2792727468 creator A5040998305 @default.
• W2792727468 creator A5051397959 @default.
• W2792727468 creator A5055515634 @default.
• W2792727468 creator A5074156162 @default.
• W2792727468 date "2018-03-07" @default.
• W2792727468 modified "2023-10-16" @default.
• W2792727468 title "Layered Structure and Complex Mechanochemistry Underlie Strength and Versatility in a Bacterial Adhesive" @default.
• W2792727468 cites W1495831881 @default.
• W2792727468 cites W1751810238 @default.
• W2792727468 cites W179372779 @default.
• W2792727468 cites W1828346848 @default.
• W2792727468 cites W1893801608 @default.
• W2792727468 cites W1936606969 @default.
• W2792727468 cites W1936624937 @default.
• W2792727468 cites W1964242194 @default.
• W2792727468 cites W1966197739 @default.
• W2792727468 cites W1968701196 @default.
• W2792727468 cites W1976310775 @default.
• W2792727468 cites W1976520210 @default.
• W2792727468 cites W1979599653 @default.
• W2792727468 cites W1980290914 @default.
• W2792727468 cites W1981966301 @default.
• W2792727468 cites W1992533162 @default.
• W2792727468 cites W1995962299 @default.
• W2792727468 cites W1999160007 @default.
• W2792727468 cites W2001847339 @default.
• W2792727468 cites W2008734023 @default.
• W2792727468 cites W2010227280 @default.
• W2792727468 cites W2011767059 @default.
• W2792727468 cites W2021152538 @default.
• W2792727468 cites W2021419038 @default.
• W2792727468 cites W2021915103 @default.
• W2792727468 cites W2027425031 @default.
• W2792727468 cites W2028831558 @default.
• W2792727468 cites W2030732731 @default.
• W2792727468 cites W2037552111 @default.
• W2792727468 cites W2044765734 @default.
• W2792727468 cites W2047205370 @default.
• W2792727468 cites W2047541630 @default.
• W2792727468 cites W2061994684 @default.
• W2792727468 cites W2063838098 @default.
• W2792727468 cites W2067835701 @default.
• W2792727468 cites W2069697378 @default.
• W2792727468 cites W2077670953 @default.
• W2792727468 cites W2096678231 @default.
• W2792727468 cites W2097007758 @default.
• W2792727468 cites W2102075418 @default.
• W2792727468 cites W2103135308 @default.
• W2792727468 cites W2103511109 @default.
• W2792727468 cites W2104748964 @default.
• W2792727468 cites W2106059993 @default.
• W2792727468 cites W2108687246 @default.
• W2792727468 cites W2110569167 @default.
• W2792727468 cites W2111693948 @default.
• W2792727468 cites W2112394563 @default.
• W2792727468 cites W2113192692 @default.
• W2792727468 cites W2115170090 @default.
• W2792727468 cites W2124817079 @default.
• W2792727468 cites W2125649895 @default.
• W2792727468 cites W2125684653 @default.
• W2792727468 cites W2129512185 @default.
• W2792727468 cites W2137364135 @default.
• W2792727468 cites W2140455148 @default.
• W2792727468 cites W2144633240 @default.
• W2792727468 cites W2147027871 @default.
• W2792727468 cites W2154713961 @default.
• W2792727468 cites W2161246773 @default.
• W2792727468 cites W2163504106 @default.
• W2792727468 cites W2168814466 @default.
• W2792727468 cites W2169418553 @default.
• W2792727468 cites W2172194338 @default.
• W2792727468 cites W2216044891 @default.
• W2792727468 cites W2271554085 @default.
• W2792727468 cites W2271558265 @default.
• W2792727468 cites W2283231348 @default.
• W2792727468 cites W2399812463 @default.
• W2792727468 cites W2416337535 @default.
• W2792727468 cites W2531651920 @default.
• W2792727468 cites W2541674309 @default.
• W2792727468 cites W2544993595 @default.
• W2792727468 cites W2562668452 @default.
• W2792727468 cites W2564351147 @default.
• W2792727468 cites W2582215292 @default.
• W2792727468 cites W2599178936 @default.
• W2792727468 cites W2604286980 @default.
• W2792727468 cites W2611419793 @default.
• W2792727468 cites W2750919021 @default.
• W2792727468 cites W2774955072 @default.
• W2792727468 cites W4250546210 @default.
• W2792727468 doi "https://doi.org/10.1128/mbio.02359-17" @default.
• W2792727468 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5801468" @default.
• W2792727468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29437925" @default.
• W2792727468 hasPublicationYear "2018" @default.
• W2792727468 type Work @default.
• W2792727468 sameAs 2792727468 @default.

• next