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- W2792796541 abstract "Background The patch test is the standard procedure for diagnosing delayed‐type sensitization. If a patch test is not possible, the flow cytometric lymphocyte proliferation test (LPT), which determines the number and type of cells responding to a specific antigen in vitro, might be considered as an alternative. Objectives Our aim was to establish a flow cytometric LPT for the detection of delayed‐type allergic responses to cobalt, and to determine the correlation between stimulation indices (SIs) in LPT and the grade of patch test reactions. With the patch test as a diagnostic reference, we also assessed the sensitivity and specificity of the LPT. Methods Fifty‐four patients patch tested with the baseline series including cobalt (CoCl 2 ) were additionally tested with the flow cytometric LPT with CoCl 2 . Results There was a significant correlation between the results of both tests: r s = 0.43; P = .001. The LPT with CoCl 2 showed a sensitivity of 52.6% and a specificity of 85.7%. Corresponding to the low sensitivity of the LPT, high likelihood ratios for a positive patch test reaction were reached only in cases of strong lymphocyte proliferation (SI ≥ 10). Conclusions In cases of clearly increased SIs, the flow cytometric LPT with CoCl 2 gives relevant diagnostic information, and represents a valuable alternative to patch testing." @default.
- W2792796541 created "2018-03-29" @default.
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- W2792796541 date "2018-03-26" @default.
- W2792796541 modified "2023-09-26" @default.
- W2792796541 title "Delayed-type allergy to cobalt-comparison of a flow cytometric lymphocyte proliferation test with patch testing" @default.
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- W2792796541 doi "https://doi.org/10.1111/cod.12990" @default.
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