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- W2792862308 abstract "Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters." @default.
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- W2792862308 date "2018-01-01" @default.
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- W2792862308 title "Purification of Fluorescent Labeled Cells from Dissociated Ciona Embryos" @default.
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- W2792862308 doi "https://doi.org/10.1007/978-981-10-7545-2_9" @default.
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