Matches in SemOpenAlex for { <https://semopenalex.org/work/W2793273466> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2793273466 endingPage "246a" @default.
- W2793273466 startingPage "246a" @default.
- W2793273466 abstract "Advances in cryo-electron microscopy have opened up new avenues to structurally define biomolecular assemblies. To arrive at detailed psedoatomic models, it is necessary to employ integrative computational modeling. Here, we illustrate the success of this hybrid structural approach in describing pre-initiation complexes of eukaryotic RNA Polymerases I and II (Pol I and Pol II) at near atomic resolution. During initiation Pol II and general transcription factors (GTFs) assemble into a pre-initiation complex (PIC). After assembly, the initial closed promoter complex (CC) transitions into an open complex (OC), in which the melted template DNA is inserted into the Pol II active site. The OC is eventually converted into an initial transcribing complex (ITC), competent to synthesize RNA. Atomic resolution description of this complex machinery had proved elusive. Combining cryo-EM with state-of-the-art computation allowed visualization of the Pol II PIC at all major stages of transcription initiation. Comparisons of the functional states provided new mechanistic insights into the processes of DNA engagement, promoter melting and transcription bubble stabilization. By contrast, Pol I transcription initiation depends on the interaction of Pol I with the GTF Core Factor (CF). Visualizing the Pol I PIC showed that Pol I and promoter DNA are a preconditioned in an elongation competent form. The structures also revealed a bipartite architecture of the Core Factor and its specific recognition of the promoter DNA. In this study the Pol I PIC was captured in three distinct functional states. Comparison of these states with the Pol II system suggests that a ratchet motion of the CF bound to the upstream DNA facilitates promoter melting in an ATP-independent manner." @default.
- W2793273466 created "2018-03-29" @default.
- W2793273466 creator A5006718110 @default.
- W2793273466 creator A5024544328 @default.
- W2793273466 creator A5034946559 @default.
- W2793273466 creator A5036415725 @default.
- W2793273466 date "2018-02-01" @default.
- W2793273466 modified "2023-09-26" @default.
- W2793273466 title "Electron Microscopy and Integrative Modeling Shed Light on the Mechanisms of Transcription Initiation" @default.
- W2793273466 doi "https://doi.org/10.1016/j.bpj.2017.11.1368" @default.
- W2793273466 hasPublicationYear "2018" @default.
- W2793273466 type Work @default.
- W2793273466 sameAs 2793273466 @default.
- W2793273466 citedByCount "0" @default.
- W2793273466 crossrefType "journal-article" @default.
- W2793273466 hasAuthorship W2793273466A5006718110 @default.
- W2793273466 hasAuthorship W2793273466A5024544328 @default.
- W2793273466 hasAuthorship W2793273466A5034946559 @default.
- W2793273466 hasAuthorship W2793273466A5036415725 @default.
- W2793273466 hasBestOaLocation W27932734661 @default.
- W2793273466 hasConcept C101762097 @default.
- W2793273466 hasConcept C104317684 @default.
- W2793273466 hasConcept C121332964 @default.
- W2793273466 hasConcept C131140672 @default.
- W2793273466 hasConcept C138885662 @default.
- W2793273466 hasConcept C150194340 @default.
- W2793273466 hasConcept C154816499 @default.
- W2793273466 hasConcept C156951783 @default.
- W2793273466 hasConcept C179926584 @default.
- W2793273466 hasConcept C185592680 @default.
- W2793273466 hasConcept C2776449523 @default.
- W2793273466 hasConcept C41895202 @default.
- W2793273466 hasConcept C54355233 @default.
- W2793273466 hasConcept C552990157 @default.
- W2793273466 hasConcept C64350747 @default.
- W2793273466 hasConcept C67705224 @default.
- W2793273466 hasConcept C70721500 @default.
- W2793273466 hasConcept C70916841 @default.
- W2793273466 hasConcept C82381507 @default.
- W2793273466 hasConcept C86803240 @default.
- W2793273466 hasConcept C95444343 @default.
- W2793273466 hasConceptScore W2793273466C101762097 @default.
- W2793273466 hasConceptScore W2793273466C104317684 @default.
- W2793273466 hasConceptScore W2793273466C121332964 @default.
- W2793273466 hasConceptScore W2793273466C131140672 @default.
- W2793273466 hasConceptScore W2793273466C138885662 @default.
- W2793273466 hasConceptScore W2793273466C150194340 @default.
- W2793273466 hasConceptScore W2793273466C154816499 @default.
- W2793273466 hasConceptScore W2793273466C156951783 @default.
- W2793273466 hasConceptScore W2793273466C179926584 @default.
- W2793273466 hasConceptScore W2793273466C185592680 @default.
- W2793273466 hasConceptScore W2793273466C2776449523 @default.
- W2793273466 hasConceptScore W2793273466C41895202 @default.
- W2793273466 hasConceptScore W2793273466C54355233 @default.
- W2793273466 hasConceptScore W2793273466C552990157 @default.
- W2793273466 hasConceptScore W2793273466C64350747 @default.
- W2793273466 hasConceptScore W2793273466C67705224 @default.
- W2793273466 hasConceptScore W2793273466C70721500 @default.
- W2793273466 hasConceptScore W2793273466C70916841 @default.
- W2793273466 hasConceptScore W2793273466C82381507 @default.
- W2793273466 hasConceptScore W2793273466C86803240 @default.
- W2793273466 hasConceptScore W2793273466C95444343 @default.
- W2793273466 hasIssue "3" @default.
- W2793273466 hasLocation W27932734661 @default.
- W2793273466 hasOpenAccess W2793273466 @default.
- W2793273466 hasPrimaryLocation W27932734661 @default.
- W2793273466 hasRelatedWork W1517278511 @default.
- W2793273466 hasRelatedWork W2030907738 @default.
- W2793273466 hasRelatedWork W2063168651 @default.
- W2793273466 hasRelatedWork W2082395806 @default.
- W2793273466 hasRelatedWork W2094385715 @default.
- W2793273466 hasRelatedWork W2416845944 @default.
- W2793273466 hasRelatedWork W2487404522 @default.
- W2793273466 hasRelatedWork W3080952367 @default.
- W2793273466 hasRelatedWork W4206591058 @default.
- W2793273466 hasRelatedWork W4210899811 @default.
- W2793273466 hasVolume "114" @default.
- W2793273466 isParatext "false" @default.
- W2793273466 isRetracted "false" @default.
- W2793273466 magId "2793273466" @default.
- W2793273466 workType "article" @default.