FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2793443334> ?p ?o ?g. }

• W2793443334 endingPage "187" @default.
• W2793443334 startingPage "182" @default.
• W2793443334 abstract "The difference of electrostatic interaction between free Ru(phen)32+ and Ru(phen)32+ embedded in double strand DNA (dsDNA) to the negatively charged indium tin oxide (ITO) electrode has been applied to develop a homogeneous and label-free electrochemiluminescence (ECL) aptasensor for the first time. Ochratoxin A (OTA) has been chosen as the model target. The OTA aptamer is first hybridized with its complementary single strand DNA (ssDNA) to form dsDNA and then interacted with Ru(phen)32+ via the grooves binding mode to form dsDNA-Ru(phen)32+ complex, which remains negatively charged feature as well as low diffusion capacity to the negatively charged ITO electrode surface owing to the electrostatic repulsion. Meanwhile, the intercalated Ru(phen)32+ in the grooves of dsDNA works as an ECL signal reporter instead of the labor-intensive labeling steps and can generate much more ECL signal than that from the labeling probe. In the presence of target, the aptamer prefers to form an aptamer-target complex in lieu of dsDNA, which induces the releasing of Ru(phen)32+ from the dsDNA-Ru(phen)32+ complex into the solution. With the assistance of RecJf exonuclease (a ssDNA specific exonuclease), the released ssDNA and the aptamer in the target-complex were digested into mononucleotides. In the meantime, the target can be also liberated from OTA-aptamer complex and induce target cycling and large amount of free Ru(phen)32+ present in the solution. Since Ru(phen)32+ contains positive charges, which can diffuses easily to the ITO electrode surface because of electrostatic attraction, causing an obviously enhanced ECL signal detected. Under the optimal conditions, the enhanced ECL of the system has a linear relationship with the OTA concentration in the range of 0.01-1.0 ng/mL with a detection limit of 2 pg/mL. This innovative system not only expands the immobilization-free sensors in the electrochemiluminescent fields, but also can be developed for the detection of different targets easily with the same strategy by changing the aptamer used." @default.
• W2793443334 created "2018-03-29" @default.
• W2793443334 creator A5000602292 @default.
• W2793443334 creator A5002488080 @default.
• W2793443334 creator A5019754443 @default.
• W2793443334 creator A5026605890 @default.
• W2793443334 creator A5041274864 @default.
• W2793443334 creator A5056448938 @default.
• W2793443334 creator A5059107046 @default.
• W2793443334 creator A5085764973 @default.
• W2793443334 date "2018-05-01" @default.
• W2793443334 modified "2023-10-14" @default.
• W2793443334 title "Homogeneous and label-free electrochemiluminescence aptasensor based on the difference of electrostatic interaction and exonuclease-assisted target recycling amplification" @default.
• W2793443334 cites W1053809098 @default.
• W2793443334 cites W1141462211 @default.
• W2793443334 cites W1850250041 @default.
• W2793443334 cites W1965763683 @default.
• W2793443334 cites W1966753544 @default.
• W2793443334 cites W1993259011 @default.
• W2793443334 cites W1994014422 @default.
• W2793443334 cites W1997307787 @default.
• W2793443334 cites W1999599585 @default.
• W2793443334 cites W2008262280 @default.
• W2793443334 cites W2010544055 @default.
• W2793443334 cites W2011611662 @default.
• W2793443334 cites W2011871731 @default.
• W2793443334 cites W2016839980 @default.
• W2793443334 cites W2038495323 @default.
• W2793443334 cites W2038582507 @default.
• W2793443334 cites W2039667141 @default.
• W2793443334 cites W2043826407 @default.
• W2793443334 cites W2068376913 @default.
• W2793443334 cites W2084154521 @default.
• W2793443334 cites W2122309268 @default.
• W2793443334 cites W2208138491 @default.
• W2793443334 cites W2216827241 @default.
• W2793443334 cites W2289042572 @default.
• W2793443334 cites W2319147021 @default.
• W2793443334 cites W2326230425 @default.
• W2793443334 cites W2329453615 @default.
• W2793443334 cites W2467424267 @default.
• W2793443334 cites W2510593474 @default.
• W2793443334 cites W2516522516 @default.
• W2793443334 cites W2526105580 @default.
• W2793443334 cites W2528767583 @default.
• W2793443334 cites W2557451414 @default.
• W2793443334 cites W2559769268 @default.
• W2793443334 cites W2575746120 @default.
• W2793443334 cites W2581104150 @default.
• W2793443334 cites W2587444579 @default.
• W2793443334 cites W2598427441 @default.
• W2793443334 cites W659228908 @default.
• W2793443334 cites W4211087902 @default.
• W2793443334 doi "https://doi.org/10.1016/j.bios.2018.01.043" @default.
• W2793443334 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29412943" @default.
• W2793443334 hasPublicationYear "2018" @default.
• W2793443334 type Work @default.
• W2793443334 sameAs 2793443334 @default.
• W2793443334 citedByCount "44" @default.
• W2793443334 countsByYear W27934433342018 @default.
• W2793443334 countsByYear W27934433342019 @default.
• W2793443334 countsByYear W27934433342020 @default.
• W2793443334 countsByYear W27934433342021 @default.
• W2793443334 countsByYear W27934433342022 @default.
• W2793443334 countsByYear W27934433342023 @default.
• W2793443334 crossrefType "journal-article" @default.
• W2793443334 hasAuthorship W2793443334A5000602292 @default.
• W2793443334 hasAuthorship W2793443334A5002488080 @default.
• W2793443334 hasAuthorship W2793443334A5019754443 @default.
• W2793443334 hasAuthorship W2793443334A5026605890 @default.
• W2793443334 hasAuthorship W2793443334A5041274864 @default.
• W2793443334 hasAuthorship W2793443334A5056448938 @default.
• W2793443334 hasAuthorship W2793443334A5059107046 @default.
• W2793443334 hasAuthorship W2793443334A5085764973 @default.
• W2793443334 hasConcept C104317684 @default.
• W2793443334 hasConcept C111368507 @default.
• W2793443334 hasConcept C127313418 @default.
• W2793443334 hasConcept C128972844 @default.
• W2793443334 hasConcept C147789679 @default.
• W2793443334 hasConcept C17525397 @default.
• W2793443334 hasConcept C185592680 @default.
• W2793443334 hasConcept C21951064 @default.
• W2793443334 hasConcept C2777289219 @default.
• W2793443334 hasConcept C2777501222 @default.
• W2793443334 hasConcept C2781243718 @default.
• W2793443334 hasConcept C32737372 @default.
• W2793443334 hasConcept C38965407 @default.
• W2793443334 hasConcept C54355233 @default.
• W2793443334 hasConcept C547475151 @default.
• W2793443334 hasConcept C552990157 @default.
• W2793443334 hasConcept C55493867 @default.
• W2793443334 hasConcept C82381507 @default.
• W2793443334 hasConcept C86803240 @default.
• W2793443334 hasConceptScore W2793443334C104317684 @default.
• W2793443334 hasConceptScore W2793443334C111368507 @default.
• W2793443334 hasConceptScore W2793443334C127313418 @default.
• W2793443334 hasConceptScore W2793443334C128972844 @default.
• W2793443334 hasConceptScore W2793443334C147789679 @default.

• next