FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2793452048> ?p ?o ?g. }

• W2793452048 abstract "Background: Metastatic breast cancer (MBC) and the circulating cells (CTCs) leading to macrometastasis are inherently different than primary breast cancer, evolving under the selection pressure of systemic therapy. A better understanding of the tumor biology of CTCs compared to metastasis may shed light on treatment opportunities. Methods: We performed whole transcriptome sequencing (RNA Seq) on fresh metastatic tumor biopsies (mets), CTCs, and peripheral blood (PB) from 21 newly diagnosed MBC patients. CTCs were harvested using the ANGLE Parsortix to isolate cells based on size and deformability. Data were analyzed for differential expression, pathways, single nucleotide variants (SNV), fusions, intrinsic subtype, and a CTC-mets shared gene signature was validated using data from The Cancer Genome Atlas (TCGA). Detailed clinical-pathological and treatment data was evaluated. Results: CTCs as a group showed much stronger gene expression of oncogenes, stem cell genes, keratins and mesenchymal markers than did mets from the same patients. Matched patient comparisons for 66 potentially clinically actionable genes for 8/9 pathways showed no significant difference in gene expression targets between CTCs and mets on ANOVA, although fold-change did vary. Eight SNVs in the ESR1 gene (n=5 patients) and 5 SNVs in the HER2 gene (n=2 patients) were shared between CTCs and distant metastases. Differential gene expression analysis identified a signature of 8870 genes that were statistically significantly correlated between CTCs and mets (FDR adjusted p Four of 21 CTC samples showed strong whole transcriptome RPKM correlation with PB (R2)>0.9, however, 3/21 CTC samples showed strong whole transcriptome RPKM correlation with mets (R2)>0.8. The remainder showed low correlation with both. Coverage was 91.4X for CTCs, 140.2X for mets and 138.5X for PB. Conclusions: We present the transcriptomic landscape of CTCs with comparison to metastases and peripheral blood all acquired prior to treatment of newly diagnosed Stage IV breast cancer. Multiple genes, including oncogenes and stem cell genes, were found with higher expression in CTCs versus metastases. When focusing on 66 known potentially clinically actionable genes in breast cancer, CTCs did not show significantly different patterns of expression than mets in terms of up-regulation versus down-regulation compared to PB. RNA Seq of CTCs may be utilized to identify molecular alterations that are potentially clinically actionable. Citation Format: Ring A, Porras T, Campo D, Kaur P, Forte VA, Tripathy D, Lu J, Zada G, Wagle N, Wecsler JS, Lang JE. The whole transcriptional landscape of circulating tumor cells compared to metastases in stage IV breast cancer [abstract]. In: Proceedings of the 2017 San Antonio Breast Cancer Symposium; 2017 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2018;78(4 Suppl):Abstract nr P2-01-04." @default.
• W2793452048 created "2018-03-29" @default.
• W2793452048 creator A5006955201 @default.
• W2793452048 creator A5023674071 @default.
• W2793452048 creator A5029436679 @default.
• W2793452048 creator A5035365137 @default.
• W2793452048 creator A5046114040 @default.
• W2793452048 creator A5048080578 @default.
• W2793452048 creator A5053462385 @default.
• W2793452048 creator A5062528111 @default.
• W2793452048 creator A5067913114 @default.
• W2793452048 creator A5083794797 @default.
• W2793452048 creator A5085362780 @default.
• W2793452048 date "2018-02-14" @default.
• W2793452048 modified "2023-09-27" @default.
• W2793452048 title "Abstract P2-01-04: The whole transcriptional landscape of circulating tumor cells compared to metastases in stage IV breast cancer" @default.
• W2793452048 doi "https://doi.org/10.1158/1538-7445.sabcs17-p2-01-04" @default.
• W2793452048 hasPublicationYear "2018" @default.
• W2793452048 type Work @default.
• W2793452048 sameAs 2793452048 @default.
• W2793452048 citedByCount "0" @default.
• W2793452048 crossrefType "proceedings-article" @default.
• W2793452048 hasAuthorship W2793452048A5006955201 @default.
• W2793452048 hasAuthorship W2793452048A5023674071 @default.
• W2793452048 hasAuthorship W2793452048A5029436679 @default.
• W2793452048 hasAuthorship W2793452048A5035365137 @default.
• W2793452048 hasAuthorship W2793452048A5046114040 @default.
• W2793452048 hasAuthorship W2793452048A5048080578 @default.
• W2793452048 hasAuthorship W2793452048A5053462385 @default.
• W2793452048 hasAuthorship W2793452048A5062528111 @default.
• W2793452048 hasAuthorship W2793452048A5067913114 @default.
• W2793452048 hasAuthorship W2793452048A5083794797 @default.
• W2793452048 hasAuthorship W2793452048A5085362780 @default.
• W2793452048 hasConcept C104317684 @default.
• W2793452048 hasConcept C121608353 @default.
• W2793452048 hasConcept C126322002 @default.
• W2793452048 hasConcept C143998085 @default.
• W2793452048 hasConcept C150194340 @default.
• W2793452048 hasConcept C162317418 @default.
• W2793452048 hasConcept C2775930923 @default.
• W2793452048 hasConcept C2779013556 @default.
• W2793452048 hasConcept C502942594 @default.
• W2793452048 hasConcept C530470458 @default.
• W2793452048 hasConcept C54355233 @default.
• W2793452048 hasConcept C61238886 @default.
• W2793452048 hasConcept C71924100 @default.
• W2793452048 hasConcept C86803240 @default.
• W2793452048 hasConceptScore W2793452048C104317684 @default.
• W2793452048 hasConceptScore W2793452048C121608353 @default.
• W2793452048 hasConceptScore W2793452048C126322002 @default.
• W2793452048 hasConceptScore W2793452048C143998085 @default.
• W2793452048 hasConceptScore W2793452048C150194340 @default.
• W2793452048 hasConceptScore W2793452048C162317418 @default.
• W2793452048 hasConceptScore W2793452048C2775930923 @default.
• W2793452048 hasConceptScore W2793452048C2779013556 @default.
• W2793452048 hasConceptScore W2793452048C502942594 @default.
• W2793452048 hasConceptScore W2793452048C530470458 @default.
• W2793452048 hasConceptScore W2793452048C54355233 @default.
• W2793452048 hasConceptScore W2793452048C61238886 @default.
• W2793452048 hasConceptScore W2793452048C71924100 @default.
• W2793452048 hasConceptScore W2793452048C86803240 @default.
• W2793452048 hasLocation W27934520481 @default.
• W2793452048 hasOpenAccess W2793452048 @default.
• W2793452048 hasPrimaryLocation W27934520481 @default.
• W2793452048 hasRelatedWork W1668195681 @default.
• W2793452048 hasRelatedWork W1997391976 @default.
• W2793452048 hasRelatedWork W2064082549 @default.
• W2793452048 hasRelatedWork W2155545746 @default.
• W2793452048 hasRelatedWork W2312404360 @default.
• W2793452048 hasRelatedWork W2343095581 @default.
• W2793452048 hasRelatedWork W2492962145 @default.
• W2793452048 hasRelatedWork W2562843546 @default.
• W2793452048 hasRelatedWork W2563805996 @default.
• W2793452048 hasRelatedWork W2593884313 @default.
• W2793452048 hasRelatedWork W2785454704 @default.
• W2793452048 hasRelatedWork W2887792120 @default.
• W2793452048 hasRelatedWork W2944798701 @default.
• W2793452048 hasRelatedWork W2946321576 @default.
• W2793452048 hasRelatedWork W2955194941 @default.
• W2793452048 hasRelatedWork W2956105004 @default.
• W2793452048 hasRelatedWork W2960994295 @default.
• W2793452048 hasRelatedWork W3012134432 @default.
• W2793452048 hasRelatedWork W3036801411 @default.
• W2793452048 hasRelatedWork W3076412310 @default.
• W2793452048 isParatext "false" @default.
• W2793452048 isRetracted "false" @default.
• W2793452048 magId "2793452048" @default.
• W2793452048 workType "article" @default.