Matches in SemOpenAlex for { <https://semopenalex.org/work/W2793485153> ?p ?o ?g. }
- W2793485153 endingPage "e0193882" @default.
- W2793485153 startingPage "e0193882" @default.
- W2793485153 abstract "The rhythmic nature of insulin secretion over the 24h cycle in pancreatic islets has been mostly investigated using transcriptomics studies showing that modulation of insulin secretion over this cycle is achieved via distal stages of insulin secretion. We set out to measure β-cell exocytosis using in depth cell physiology techniques at several time points. In agreement with the activity and feeding pattern of nocturnal rodents, we find that C57/Bl6J islets in culture for 24h exhibit higher insulin secretion during the corresponding dark phase than in the light phase (Zeitgeber Time ZT20 and ZT8, respectively, in vivo). Glucose-induced insulin secretion is increased by 21% despite normal intracellular Ca2+ transients and depolarization-evoked exocytosis, as measured by whole-cell capacitance measurements. This paradox is explained by a 1.37-fold increase in beta cell insulin content. Ultramorphological analyses show that vesicle size and density are unaltered, demonstrating that intravesicular insulin content per granule is modulated over the 24h cycle. Proinsulin levels did not change between ZT8 and ZT20. Islet glucagon content was inversely proportional to insulin content indicating that this unique feature is likely to support a physiological role. Microarray data identified the differential expression of 301 transcripts, of which 26 are miRNAs and 54 are known genes (including C2cd4b, a gene previously involved in insulin processing, and clock genes such as Bmal1 and Rev-erbα). Mouse β-cell secretion over the full course of the 24h cycle may rely on several distinct cellular functions but late night increase in insulin secretion depends solely on granule insulin content." @default.
- W2793485153 created "2018-03-29" @default.
- W2793485153 creator A5004803054 @default.
- W2793485153 creator A5005538317 @default.
- W2793485153 creator A5011038491 @default.
- W2793485153 creator A5014708404 @default.
- W2793485153 creator A5016547262 @default.
- W2793485153 creator A5024329533 @default.
- W2793485153 creator A5031245985 @default.
- W2793485153 creator A5075721124 @default.
- W2793485153 creator A5080584624 @default.
- W2793485153 creator A5087399727 @default.
- W2793485153 creator A5087807982 @default.
- W2793485153 date "2018-03-15" @default.
- W2793485153 modified "2023-10-18" @default.
- W2793485153 title "Modulation of large dense core vesicle insulin content mediates rhythmic hormone release from pancreatic beta cells over the 24h cycle" @default.
- W2793485153 cites W1826492881 @default.
- W2793485153 cites W1932256504 @default.
- W2793485153 cites W1935468480 @default.
- W2793485153 cites W1967142993 @default.
- W2793485153 cites W1987546711 @default.
- W2793485153 cites W2014780202 @default.
- W2793485153 cites W2037347257 @default.
- W2793485153 cites W2074367229 @default.
- W2793485153 cites W2075091287 @default.
- W2793485153 cites W2079466432 @default.
- W2793485153 cites W2093478842 @default.
- W2793485153 cites W2104382389 @default.
- W2793485153 cites W2108359398 @default.
- W2793485153 cites W2128342607 @default.
- W2793485153 cites W2140402299 @default.
- W2793485153 cites W2140464228 @default.
- W2793485153 cites W2147712103 @default.
- W2793485153 cites W2162650381 @default.
- W2793485153 cites W2165080214 @default.
- W2793485153 cites W2171750333 @default.
- W2793485153 cites W2175535734 @default.
- W2793485153 cites W2195673264 @default.
- W2793485153 cites W2196732710 @default.
- W2793485153 cites W2237255498 @default.
- W2793485153 cites W2304342549 @default.
- W2793485153 cites W2468942193 @default.
- W2793485153 cites W2609892848 @default.
- W2793485153 doi "https://doi.org/10.1371/journal.pone.0193882" @default.
- W2793485153 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5854349" @default.
- W2793485153 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29543849" @default.
- W2793485153 hasPublicationYear "2018" @default.
- W2793485153 type Work @default.
- W2793485153 sameAs 2793485153 @default.
- W2793485153 citedByCount "3" @default.
- W2793485153 countsByYear W27934851532020 @default.
- W2793485153 countsByYear W27934851532022 @default.
- W2793485153 crossrefType "journal-article" @default.
- W2793485153 hasAuthorship W2793485153A5004803054 @default.
- W2793485153 hasAuthorship W2793485153A5005538317 @default.
- W2793485153 hasAuthorship W2793485153A5011038491 @default.
- W2793485153 hasAuthorship W2793485153A5014708404 @default.
- W2793485153 hasAuthorship W2793485153A5016547262 @default.
- W2793485153 hasAuthorship W2793485153A5024329533 @default.
- W2793485153 hasAuthorship W2793485153A5031245985 @default.
- W2793485153 hasAuthorship W2793485153A5075721124 @default.
- W2793485153 hasAuthorship W2793485153A5080584624 @default.
- W2793485153 hasAuthorship W2793485153A5087399727 @default.
- W2793485153 hasAuthorship W2793485153A5087807982 @default.
- W2793485153 hasBestOaLocation W27934851531 @default.
- W2793485153 hasConcept C121446783 @default.
- W2793485153 hasConcept C126322002 @default.
- W2793485153 hasConcept C134018914 @default.
- W2793485153 hasConcept C144855686 @default.
- W2793485153 hasConcept C165220095 @default.
- W2793485153 hasConcept C175969161 @default.
- W2793485153 hasConcept C21786239 @default.
- W2793485153 hasConcept C2776828364 @default.
- W2793485153 hasConcept C2777391703 @default.
- W2793485153 hasConcept C2779306644 @default.
- W2793485153 hasConcept C2779855799 @default.
- W2793485153 hasConcept C3018667095 @default.
- W2793485153 hasConcept C38606739 @default.
- W2793485153 hasConcept C49039625 @default.
- W2793485153 hasConcept C71924100 @default.
- W2793485153 hasConcept C86803240 @default.
- W2793485153 hasConceptScore W2793485153C121446783 @default.
- W2793485153 hasConceptScore W2793485153C126322002 @default.
- W2793485153 hasConceptScore W2793485153C134018914 @default.
- W2793485153 hasConceptScore W2793485153C144855686 @default.
- W2793485153 hasConceptScore W2793485153C165220095 @default.
- W2793485153 hasConceptScore W2793485153C175969161 @default.
- W2793485153 hasConceptScore W2793485153C21786239 @default.
- W2793485153 hasConceptScore W2793485153C2776828364 @default.
- W2793485153 hasConceptScore W2793485153C2777391703 @default.
- W2793485153 hasConceptScore W2793485153C2779306644 @default.
- W2793485153 hasConceptScore W2793485153C2779855799 @default.
- W2793485153 hasConceptScore W2793485153C3018667095 @default.
- W2793485153 hasConceptScore W2793485153C38606739 @default.
- W2793485153 hasConceptScore W2793485153C49039625 @default.
- W2793485153 hasConceptScore W2793485153C71924100 @default.
- W2793485153 hasConceptScore W2793485153C86803240 @default.
- W2793485153 hasIssue "3" @default.