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- W2793552239 abstract "A central methodological aim of the biosciences is to experimentally simulate, as good as possible, the real environmental conditions of biological systems in order to gain a true image of the effects and processes of interest. Thus, the bioscientific research is moving from test-tube observations towards in vivo investigations in intact cells and tissues. In this frame, the development of biophysical techniques, which allow an accurate and highly sensitive registration of changes in the studied system without disturbing the natural processes therein, is particularly significant. Especially the fluorescence laser microscopy provide a large diversity of versatile tools for bioscientific studies. In this work, two new theoretical approaches are developed, which allow an extended comprehension of the single-molecule fluorescence fluctuation microscopy based on two-photon excitation, one of the most sensitive techniques for biological investigations. Moreover, novel non-invasive fluorescence lifetime imaging experiments are performed, which enable us a detailed and accurate insight in the cellular NAD(P)H-metabolism and in the functionality of artificial skin as an organ." @default.
- W2793552239 created "2018-03-29" @default.
- W2793552239 creator A5078207655 @default.
- W2793552239 date "2005-01-10" @default.
- W2793552239 modified "2023-09-23" @default.
- W2793552239 title "New advancements in highly sensitive time-resolved fluorescence two-photon microscopy: Theoretical approaches and bioscientific applications" @default.
- W2793552239 doi "https://doi.org/10.24355/dbbs.084-200511080100-344" @default.
- W2793552239 hasPublicationYear "2005" @default.
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