FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2793559179> ?p ?o ?g. }

• W2793559179 endingPage "50" @default.
• W2793559179 startingPage "42" @default.
• W2793559179 abstract "ObjectivesOrientia tsutsugamushi is an obligate intracellular pathogen that causes scrub typhus. Diagnosing scrub typhus remains a challenge, and a sensitive, specific, simple, and rapid diagnostic test is still needed.MethodsA recombinase polymerase amplification (RPA) assay combined with a lateral flow (LF) test targeting the 56-kDa gene of a Karp-like strain of O. tsutsugamushi was developed and optimized. The detection limits, sensitivity, specificity, and simulative clinical performance were evaluated.ResultsPrimers and probe were screened to establish the RPA assay, and the reaction conditions were optimized. The detection limit was 10 copies/reaction in detecting plasmid DNA and 12 copies/reaction in detecting genomic DNA. The RPA–LF method could differentiate O. tsutsugamushi from other phylogenetically related bacteria. The sensitivity was 100% and specificity was over 90%, when evaluated using infected animal samples or simulative clinical samples. Furthermore, the method was completed in 20 min at 37 °C followed by a 3–5 min incubation at room temperature for the development of an immunochromatographic strip, and the results could be determined visually.ConclusionsThis method is promising for wide-ranging use in basic medical units considering that it requires minimal instruments and infrastructure and is highly time-efficient, sensitive, and specific for diagnosing scrub typhus." @default.
• W2793559179 created "2018-03-29" @default.
• W2793559179 creator A5008598196 @default.
• W2793559179 creator A5010999092 @default.
• W2793559179 creator A5025361932 @default.
• W2793559179 creator A5031654301 @default.
• W2793559179 creator A5032162374 @default.
• W2793559179 creator A5041151940 @default.
• W2793559179 creator A5045262172 @default.
• W2793559179 creator A5053783274 @default.
• W2793559179 creator A5054724934 @default.
• W2793559179 creator A5061860314 @default.
• W2793559179 creator A5063729830 @default.
• W2793559179 creator A5067963962 @default.
• W2793559179 creator A5078354018 @default.
• W2793559179 creator A5078792881 @default.
• W2793559179 creator A5079974540 @default.
• W2793559179 creator A5084456806 @default.
• W2793559179 creator A5085192101 @default.
• W2793559179 date "2018-05-01" @default.
• W2793559179 modified "2023-10-06" @default.
• W2793559179 title "Development of a rapid and visual nucleotide detection method for a Chinese epidemic strain of Orientia tsutsugamushi based on recombinase polymerase amplification assay and lateral flow test" @default.
• W2793559179 cites W1830754368 @default.
• W2793559179 cites W1965944581 @default.
• W2793559179 cites W1969325889 @default.
• W2793559179 cites W1973249339 @default.
• W2793559179 cites W2001787096 @default.
• W2793559179 cites W2017282682 @default.
• W2793559179 cites W2092325597 @default.
• W2793559179 cites W2106807209 @default.
• W2793559179 cites W2116649218 @default.
• W2793559179 cites W2151305635 @default.
• W2793559179 cites W2153350094 @default.
• W2793559179 cites W2765292199 @default.
• W2793559179 cites W894275280 @default.
• W2793559179 doi "https://doi.org/10.1016/j.ijid.2018.03.003" @default.
• W2793559179 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29548879" @default.
• W2793559179 hasPublicationYear "2018" @default.
• W2793559179 type Work @default.
• W2793559179 sameAs 2793559179 @default.
• W2793559179 citedByCount "13" @default.
• W2793559179 countsByYear W27935591792018 @default.
• W2793559179 countsByYear W27935591792019 @default.
• W2793559179 countsByYear W27935591792020 @default.
• W2793559179 countsByYear W27935591792021 @default.
• W2793559179 countsByYear W27935591792022 @default.
• W2793559179 countsByYear W27935591792023 @default.
• W2793559179 crossrefType "journal-article" @default.
• W2793559179 hasAuthorship W2793559179A5008598196 @default.
• W2793559179 hasAuthorship W2793559179A5010999092 @default.
• W2793559179 hasAuthorship W2793559179A5025361932 @default.
• W2793559179 hasAuthorship W2793559179A5031654301 @default.
• W2793559179 hasAuthorship W2793559179A5032162374 @default.
• W2793559179 hasAuthorship W2793559179A5041151940 @default.
• W2793559179 hasAuthorship W2793559179A5045262172 @default.
• W2793559179 hasAuthorship W2793559179A5053783274 @default.
• W2793559179 hasAuthorship W2793559179A5054724934 @default.
• W2793559179 hasAuthorship W2793559179A5061860314 @default.
• W2793559179 hasAuthorship W2793559179A5063729830 @default.
• W2793559179 hasAuthorship W2793559179A5067963962 @default.
• W2793559179 hasAuthorship W2793559179A5078354018 @default.
• W2793559179 hasAuthorship W2793559179A5078792881 @default.
• W2793559179 hasAuthorship W2793559179A5079974540 @default.
• W2793559179 hasAuthorship W2793559179A5084456806 @default.
• W2793559179 hasAuthorship W2793559179A5085192101 @default.
• W2793559179 hasBestOaLocation W27935591791 @default.
• W2793559179 hasConcept C104317684 @default.
• W2793559179 hasConcept C105702510 @default.
• W2793559179 hasConcept C119128265 @default.
• W2793559179 hasConcept C126322002 @default.
• W2793559179 hasConcept C139713532 @default.
• W2793559179 hasConcept C153911025 @default.
• W2793559179 hasConcept C159047783 @default.
• W2793559179 hasConcept C185592680 @default.
• W2793559179 hasConcept C22744801 @default.
• W2793559179 hasConcept C2522874641 @default.
• W2793559179 hasConcept C2776460866 @default.
• W2793559179 hasConcept C2778022156 @default.
• W2793559179 hasConcept C2778434417 @default.
• W2793559179 hasConcept C2779528086 @default.
• W2793559179 hasConcept C2780414542 @default.
• W2793559179 hasConcept C2780823208 @default.
• W2793559179 hasConcept C40993552 @default.
• W2793559179 hasConcept C43617362 @default.
• W2793559179 hasConcept C49105822 @default.
• W2793559179 hasConcept C54355233 @default.
• W2793559179 hasConcept C552990157 @default.
• W2793559179 hasConcept C71924100 @default.
• W2793559179 hasConcept C86803240 @default.
• W2793559179 hasConcept C89423630 @default.
• W2793559179 hasConceptScore W2793559179C104317684 @default.
• W2793559179 hasConceptScore W2793559179C105702510 @default.
• W2793559179 hasConceptScore W2793559179C119128265 @default.
• W2793559179 hasConceptScore W2793559179C126322002 @default.
• W2793559179 hasConceptScore W2793559179C139713532 @default.
• W2793559179 hasConceptScore W2793559179C153911025 @default.
• W2793559179 hasConceptScore W2793559179C159047783 @default.
• W2793559179 hasConceptScore W2793559179C185592680 @default.

• next