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- W2793684557 abstract "Mass spectrometry (MS)-based techniques have been finding utility as sensitive, high throughput metabolite analysis tools for complex biological samples. We describe here a nanoflow liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) system we developed and applied to metabolic profiling of human cells. Metabolites are extracted from cells using methanol, and filtered through a C18 StageTip to remove large particles. Metabolite samples are separated by HPLC at a flow rate of 400-500 nl/min, then analyzed in both positive and negative ion modes in an LTQ-Orbitrap MS. Metabolite identification and differential analysis are performed using commercial or open source software. Protocols outlined in this chapter describe how nano-LC-MS can be applied to investigate metabolic profiling with limited biomass amount." @default.
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- W2793684557 date "2018-01-01" @default.
- W2793684557 modified "2023-09-26" @default.
- W2793684557 title "Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography–Tandem Mass Spectrometry" @default.
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- W2793684557 doi "https://doi.org/10.1007/978-1-4939-7659-1_10" @default.
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