Matches in SemOpenAlex for { <https://semopenalex.org/work/W279384457> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W279384457 abstract "Inflammatory bowel disease (IBD) comprises diseases that are characterized by chronic or relapsing inflammation of the gastrointestinal tract. Primary sclerosing cholangitis (PSC) is an extraintestinal manifestation in IBD. Immunoreactivity against an autoantigen that is expressed both in the gastrointestinal tract and the biliary tract could be the link between these diseases. A possible source of such an antigen is goblet cells. Immunostainings of normal human tissues using IBD patient sera showed goblet cell immunoreactivity against goblet cells in all parts of the gastrointestinal tract. The most frequent immunostaining was found against goblet cells in the appendix against which 84% (42/50) of IBD patients compared to 8% (4/50) of healthy blood donors showed immunoreactivity. To identify the corresponding antigen we used three different approaches, investigation of immunoreactivity to different candidate proteins compared to IBD sera, immunoscreening of an appendiceal cDNA library, and immunoprecipitation of protein lysates from mucin producing cells followed by SDS-PAGE and 2D gel electrophoresis. These approaches led to the identification of several candidate autoantigens of which complement C3 is the most promising. A novel staining pattern with strong immunoreactivity to granules and the apical membrane of biliary epithelial cells was identified with 35% (12/34) of PSC sera compared to none of healthy controls (n=28). Screening of a cDNA library from normal human choledochus identified PDZ domain containing 1 (Pdzk1) and Glutathion S transferase theta 1 (GSTT1) as potential candidates. Pdzk1 is an interesting candidate which is expressed in the intestinal tract and bile ducts. GSTT1 antibodies were not specific for PSC and are thought to develop as an alloimmune response in patients with the GSTT1-null genotype. In conclusion, we have identified specific immunoreactivity to goblet cells and biliary epithelial cells using sera from patients with IBD and PSC respectively. We have also identified several potential autoantigens." @default.
- W279384457 created "2016-06-24" @default.
- W279384457 creator A5021512169 @default.
- W279384457 creator A5028168158 @default.
- W279384457 creator A5078801536 @default.
- W279384457 creator A5080721477 @default.
- W279384457 creator A5081357043 @default.
- W279384457 creator A5084300940 @default.
- W279384457 date "2008-01-01" @default.
- W279384457 modified "2023-09-27" @default.
- W279384457 title "Immunoreactivity against bile duct epithelial cells and identification of PDZ domain containing 1 as a novel autoantigen in Primary sclerosing cholangitis." @default.
- W279384457 hasPublicationYear "2008" @default.
- W279384457 type Work @default.
- W279384457 sameAs 279384457 @default.
- W279384457 citedByCount "0" @default.
- W279384457 crossrefType "journal-article" @default.
- W279384457 hasAuthorship W279384457A5021512169 @default.
- W279384457 hasAuthorship W279384457A5028168158 @default.
- W279384457 hasAuthorship W279384457A5078801536 @default.
- W279384457 hasAuthorship W279384457A5080721477 @default.
- W279384457 hasAuthorship W279384457A5081357043 @default.
- W279384457 hasAuthorship W279384457A5084300940 @default.
- W279384457 hasConcept C126322002 @default.
- W279384457 hasConcept C142724271 @default.
- W279384457 hasConcept C147483822 @default.
- W279384457 hasConcept C159654299 @default.
- W279384457 hasConcept C179264091 @default.
- W279384457 hasConcept C203014093 @default.
- W279384457 hasConcept C204232928 @default.
- W279384457 hasConcept C2775941134 @default.
- W279384457 hasConcept C2775982439 @default.
- W279384457 hasConcept C2778260677 @default.
- W279384457 hasConcept C2779134260 @default.
- W279384457 hasConcept C2780512811 @default.
- W279384457 hasConcept C2780615123 @default.
- W279384457 hasConcept C4224716 @default.
- W279384457 hasConcept C529295009 @default.
- W279384457 hasConcept C71924100 @default.
- W279384457 hasConcept C86803240 @default.
- W279384457 hasConceptScore W279384457C126322002 @default.
- W279384457 hasConceptScore W279384457C142724271 @default.
- W279384457 hasConceptScore W279384457C147483822 @default.
- W279384457 hasConceptScore W279384457C159654299 @default.
- W279384457 hasConceptScore W279384457C179264091 @default.
- W279384457 hasConceptScore W279384457C203014093 @default.
- W279384457 hasConceptScore W279384457C204232928 @default.
- W279384457 hasConceptScore W279384457C2775941134 @default.
- W279384457 hasConceptScore W279384457C2775982439 @default.
- W279384457 hasConceptScore W279384457C2778260677 @default.
- W279384457 hasConceptScore W279384457C2779134260 @default.
- W279384457 hasConceptScore W279384457C2780512811 @default.
- W279384457 hasConceptScore W279384457C2780615123 @default.
- W279384457 hasConceptScore W279384457C4224716 @default.
- W279384457 hasConceptScore W279384457C529295009 @default.
- W279384457 hasConceptScore W279384457C71924100 @default.
- W279384457 hasConceptScore W279384457C86803240 @default.
- W279384457 hasLocation W2793844571 @default.
- W279384457 hasOpenAccess W279384457 @default.
- W279384457 hasPrimaryLocation W2793844571 @default.
- W279384457 hasRelatedWork W153659035 @default.
- W279384457 hasRelatedWork W1720076946 @default.
- W279384457 hasRelatedWork W1756522554 @default.
- W279384457 hasRelatedWork W1838841813 @default.
- W279384457 hasRelatedWork W1927092294 @default.
- W279384457 hasRelatedWork W1975738323 @default.
- W279384457 hasRelatedWork W1984487916 @default.
- W279384457 hasRelatedWork W1992693233 @default.
- W279384457 hasRelatedWork W2004169414 @default.
- W279384457 hasRelatedWork W2004505501 @default.
- W279384457 hasRelatedWork W2018800904 @default.
- W279384457 hasRelatedWork W2022835306 @default.
- W279384457 hasRelatedWork W2044068906 @default.
- W279384457 hasRelatedWork W2058032473 @default.
- W279384457 hasRelatedWork W2067265497 @default.
- W279384457 hasRelatedWork W2071826126 @default.
- W279384457 hasRelatedWork W2118122421 @default.
- W279384457 hasRelatedWork W2157892487 @default.
- W279384457 hasRelatedWork W2415773088 @default.
- W279384457 hasRelatedWork W3196789764 @default.
- W279384457 isParatext "false" @default.
- W279384457 isRetracted "false" @default.
- W279384457 magId "279384457" @default.
- W279384457 workType "article" @default.