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- W2794006944 abstract "This study describes a sensitive and fluorescent microplate assay method to detect lipase transesterification activity. Lipase-catalyzed transesterification between butyryl 4-methyl umbelliferone (Bu-4-Mu) and methanol in tert-butanol was selected as the model reaction. The release of 4-methylumbelliferone (4-Mu) in the reaction was determined by detecting the fluorescence intensity at λex 330 nm and λem 390 nm. Several lipases were used to investigate the accuracy and efficiency of the proposed method. Apparent Michaelis constant (Km) was calculated for transesterification between Bu-4-Mu and methanol by the lipases. The main advantages of the assay method include high sensitivity, inexpensive reagents, and simple detection process." @default.
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- W2794006944 date "2018-05-01" @default.
- W2794006944 modified "2023-10-17" @default.
- W2794006944 title "Fluorescent microplate assay method for high-throughput detection of lipase transesterification activity" @default.
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- W2794006944 doi "https://doi.org/10.1016/j.ab.2018.03.010" @default.
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